This paper examines the dynamics of 14 metals in water, suspended matter, sediments and hydrobionts of Dniester river in relation to natural and anthropogenic factors. The dynamics of metals in water and suspended matter of Dniester river and Dubasari reservoir depend upon the hydrologic condition, regulated by the Dniestrovsk storage reservoir. Building of the hydrotechnical constructions produced changes in the sedimentation processes, migration and distribution of metals from the newly-created storage reservoir both to Dubasari reservoir and Lower part of Dniester river. In Dniester river (Naslavcia-Otaci-Soroca sector) and Dubasari reservoir, a sharp decrease in water flow was observed, leading to an intensification of sedimentation processes of suspended metals and consequently to a high decrease in metal concentration ratio from water and suspension. Therefore, in 7-15% of samples, metal content in suspended matter was lower than in water, which was not observed before the construction of the storage reservoir Dniestrovsk. In Dubasari reservoir, in 65-85 cases metal content in water was higher than in suspended matter, while before the storage reservoir, this being characterized only for 5-11% of samples. In the last years, in Dniester river and Dubasari reservoir, the water surface is covering with aquatic plants, especially with filamentous algae. The range of metal content fluctuations in aquatic plants, in benthic invertebrates and in tissues of fish of Dniester river and Dubasari reservoir was very high, that was related not only to environmental factor variations and biological role of metals, but also to taxonomical patterns and the age of hydrobionts.