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 SM ISO690:2012IVANOVA, Raisa, BRINDZA, Ján. Changes in dyes and antioxidant capacity of pokeweed berries during storage. In: International Congress of Geneticists and Breeders from the Republic of Moldova, Ed. 11, 15-16 iunie 2021, Chişinău. Chișinău, Republica Moldova: Centrul Editorial-Poligrafic al Universităţii de Stat din Moldova, 2021, Ediția 11, p. 91. ISBN 978-9975-933-56-8. DOI: https://doi.org/10.53040/cga11.2021.069 |
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| International Congress of Geneticists and Breeders from the Republic of Moldova Ediția 11, 2021 |
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Congresul "International Congress of Geneticists and Breeders from the Republic of Moldova" 11, Chişinău, Moldova, 15-16 iunie 2021 | |
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| DOI:https://doi.org/10.53040/cga11.2021.069 | |
| CZU: 582.572.232:581.19:633.81 | |
| Pag. 91-91 | |
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Red-violet and yellow-orange dyes accumulated in berries of pokeweed (Phytolacca americana) as betacyanin – betaxanthin complex could be used as natural colorants for food, cosmetic and pharmaceutical products. The purpose of this study was to evaluate of dyes content and antioxidant capacity of juice from fresh and frozen pokeweed berries. Pokeweed berries were harvested in the seasons of 2019-2020 from P.americana plants grown at the research field station of Institute of Genetics and Plant Protection (Chisinau). The juice of fresh collected and frozen berries stored at -12°C during 6-12 months was analyzed. The content of dyes, recorded using UV-Vis spectrophotometry at 540 nm, was recalculated on two standard substances: a) betacyanin using its extinction coefficient, and b) cobalt (II) sulphate from absorbance-dose dependent curve. Antioxidant capacity of juice was studied by DPPH procedure using 1,1-diphenyl-2picrylhydrazil radicals and expressed in Trolox equivalent (TEAC). The concentration of colorant substances in juice obtained from fresh pokeweed berries was equal 2.07±0.04 g/l of betacyanins, or 14.65±0.24 g of cobalt sulphate equivalent per ml of juice. Conditions of storage did not affect the content of juice dyes, which after storage were 2.01±0.19 g/l and 14.22±0.78 g/ml, respectively in betacyanins and cobalt sulphate equivalent. However, during storage the antioxidant potential of pokeweed juices significantly decreased. The antioxidant capacity of juice from fresh berries was characterized by TEAC values of 4,41±0.51 mg/ml which after berries storage diminished by 1.8-2.5 times. It was shown significant dependences of antioxidant scavenging capacity against DPPH free radicals on total polyphenolic and hydroxycinnamic derivates contents in P.americana berries. These chemical constituents of pokeweed can easily oxidize during storage that leads to the loss of their antioxidant capacity. Natural dyes from pokeweed berries were stable during the 6-12 months of storage at -12°C. The decrease in the antioxidant potential of stored berries was conditioned by chemical constituents different from the betacyanin - betaxanthin complex. |
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Cerif XML Export
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The purpose of this study was to evaluate of dyes content and antioxidant capacity of juice from fresh and frozen pokeweed berries. Pokeweed berries were harvested in the seasons of 2019-2020 from P.americana plants grown at the research field station of Institute of Genetics and Plant Protection (Chisinau). The juice of fresh collected and frozen berries stored at -12°C during 6-12 months was analyzed. The content of dyes, recorded using UV-Vis spectrophotometry at 540 nm, was recalculated on two standard substances: a) betacyanin using its extinction coefficient, and b) cobalt (II) sulphate from absorbance-dose dependent curve. Antioxidant capacity of juice was studied by DPPH procedure using 1,1-diphenyl-2picrylhydrazil radicals and expressed in Trolox equivalent (TEAC). The concentration of colorant substances in juice obtained from fresh pokeweed berries was equal 2.07±0.04 g/l of betacyanins, or 14.65±0.24 g of cobalt sulphate equivalent per ml of juice. Conditions of storage did not affect the content of juice dyes, which after storage were 2.01±0.19 g/l and 14.22±0.78 g/ml, respectively in betacyanins and cobalt sulphate equivalent. However, during storage the antioxidant potential of pokeweed juices significantly decreased. The antioxidant capacity of juice from fresh berries was characterized by TEAC values of 4,41±0.51 mg/ml which after berries storage diminished by 1.8-2.5 times. It was shown significant dependences of antioxidant scavenging capacity against DPPH free radicals on total polyphenolic and hydroxycinnamic derivates contents in P.americana berries. These chemical constituents of pokeweed can easily oxidize during storage that leads to the loss of their antioxidant capacity. Natural dyes from pokeweed berries were stable during the 6-12 months of storage at -12°C. 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