Fabaceae species play a crucial role in natural ecosystems and agriculture, because they have the potential to symbiotic fixation of atmospheric nitrogen, improve biological activity and soil structure, increase the quality and quantity of food and feed, bring improvements resource efficiency in various biorefinery systems. We have studied biological features, biochemical composition and nutritive value, and have esteemed the biomethane potential of aerial biomass of the Fabaceae species Lupinus agustifolius and Lupinus albus, which has been cultivated in the experimental plot of the National Botanical Garden (Institute) “Alexandru Ciubotaru”, Chisinau, R. Moldova, Onobrychis viciifolia were used as control variant. The results of our research revealed that the dry matter of harvested Lupinus species whole plants contained 166-206 g/kg CP, 86-110 g/kg ash, 221258 g/kg ADF, 337-339 g/kg NDF, 31-40 g/kg ADL, 190 -218g/kg Cel and 116141 g/kg HC, but control Onobrychis viciifolia - 176 g/kg CP, 96g/kg ash, 309 g/kg ADF, 447 g/kg NDF, 49 g/kg ADL, 260 g/kg Cel and 138 g/kg HC, respectively. The nutritional value of Lupinus green mass:75.5- 80.9 % DDM, 72.9-76.6 % ODM, RFV 168-208, 13.45-13.96 MJ/kg DE, 11.04- 11.46 MJ/kg ME and 7.067.48 MJ/kg NEl in comparison with Onobrychis viciifolia - 66.9 % DDM, 61.5% ODM, RFV 142, 12.73 MJ/kg DE, 10.46 MJ/kg ME and 6.48 MJ/kg NEl. It has been found that the biomethane potential of the Lupinus substrates varied from 309 to 324 l/kg ODM. The Lupinus agustifolius and Lupinus albus is promising source of fodder and bioenergy production.