The aim of this study was to evaluate some biological peculiarities, the quality of green mass and silage from the nontraditional annual grass species Pennisetum glaucum grown in an experimental field of the National Botanical Garden (Institute), Chişinău, Republic of Moldova. It has been found that the dry matter of harvested whole plants contained 112 g/kg CP, 73 g/kg ash, 332 g/kg ADF, 591 g/kg NDF, 43 g/kg ADL; the feed value – 65.5 % DDM and 60.6% OMD with 10.19 MJ/kg ME and 6.27 MJ/kg NEl. The prepared silage was characterized by an agreeable color and a pleasant smell, with pH 4.23, it contained 17.7 g/kg DM lactic acid, 7.1 g/kg DM acetic acid, 915 g/kg organic matter, 112 g/kg CP, 337 g/kg ADF, 595 g/kg NDF, 29 g/kg ADL with 73.6 % DMD, 65.0% OMD, 11.74 MJ/kg ME and 7.07 MJ/kg NEl. The biochemical methane potential of investigated Pennisetum glaucum substrates was 337 - 360 l/kg ODM. Pennisetum glaucum contains a lot of nutrients, which make it suitable to be used as an alternative feed for livestock and feedstock for renewable energy production.