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576.75:633.85:57.085 (1) |
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SM ISO690:2012 SMEREA, Svetlana, ANDRONIC, Larisa, SCHIN, Victoria. Morphogenetic potential of calluses derived from gamma irradiated safflower seeds. . In: Oltenia - studii si comunicari stiintele naturii, 2018, nr. 2(34), pp. 67-71. ISSN 1454-6914. |
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Oltenia - studii si comunicari stiintele naturii | ||||||
Numărul 2(34) / 2018 / ISSN 1454-6914 | ||||||
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CZU: 576.75:633.85:57.085 | ||||||
Pag. 67-71 | ||||||
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In order to induce morphological diversities in safflower experimental mutagenesis was applied in combination with in vitro culture. According to the tasks of inducing somaclonal variability seeds irradiated with gamma rays in doses of 50, 100, 150 Gy were used. Two types of explants (fragments of leaves cotyledons and hypocotyls) were applied for inducing in vitro morphogenesis. As callus-inducing mineral base medium was used by MURASHIGE & SKOOG (1962) with additions of growth regulators (6Benzylaminopurine and 2,4-Dichlorophenoxyacetic acid). The derived calli were cultivated for induction of morphogenesis, been identified the hormones balance added to the culture media, the interval between subcultivation. Based on proved researches, a greater diversity in the safflower morphogenetic potential was revealed, generated by in vitro culture in a complex with gamma radiation. |
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Cuvinte-cheie in vitro morphogenesis, callus, gamma radiation, Safflower, morfogeneză in vitro, calus, gama radiație, șofrănel. |
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