<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xmlns='http://datacite.org/schema/kernel-3' xsi:schemaLocation='http://datacite.org/schema/kernel-3 http://schema.datacite.org/meta/kernel-3/metadata.xsd'>
<identifier identifierType='DOI'>10.52418/moldovan-med-j.65-2.22.04</identifier>
<creators>
<creator>
<creatorName>Ignatov, O.A.</creatorName>
<affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Cociug, A.M.</creatorName>
<affiliation>Banca de Ţesuturi Umane din Moldova, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Pascal, O.C.</creatorName>
<affiliation>Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Nacu, V.E.</creatorName>
<affiliation>Banca de Ţesuturi Umane din Moldova, Moldova, Republica</affiliation>
</creator>
</creators>
<titles>
<title xml:lang='en'>Morphological evaluation of the amniotic membrane decellularization</title>
</titles>
<publisher>Instrumentul Bibliometric National</publisher>
<publicationYear>2022</publicationYear>
<relatedIdentifier relatedIdentifierType='ISSN' relationType='IsPartOf'>2537-6373</relatedIdentifier>
<subjects>
<subject>amniotic membrane</subject>
<subject>decellularization</subject>
<subject>morphology</subject>
<subject schemeURI='http://udcdata.info/' subjectScheme='UDC'>611.013.8</subject>
</subjects>
<dates>
<date dateType='Issued'>2022-08-01</date>
</dates>
<resourceType resourceTypeGeneral='Text'>Journal article</resourceType>
<descriptions>
<description xml:lang='en' descriptionType='Abstract'><p>Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.</p></description>
</descriptions>
<formats>
<format>application/pdf</format>
</formats>
</resource>