| Conţinutul numărului revistei |
| Articolul precedent |
| Articolul urmator |
 |
 327 5 |
| Ultima descărcare din IBN: 2023-08-31 21:46 |
| Căutarea după subiecte similare conform CZU |
| 611.013.8 (6) |
| Anatomy. Human and comparative anatomy (239) |
 SM ISO690:2012IGNATOV, Olga, COCIUG, Adrian, PASCAL, Oleg, NACU, Viorel. Morphological evaluation of the amniotic membrane decellularization. In: Moldovan Medical Journal, 2022, nr. 2(65), pp. 30-35. ISSN 2537-6373. DOI: https://doi.org/10.52418/moldovan-med-j.65-2.22.04 |
| EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
| Moldovan Medical Journal | ||||||
| Numărul 2(65) / 2022 / ISSN 2537-6373 /ISSNe 2537-6381 | ||||||
|
||||||
| DOI:https://doi.org/10.52418/moldovan-med-j.65-2.22.04 | ||||||
| CZU: 611.013.8 | ||||||
| Pag. 30-35 | ||||||
|
||||||
 Descarcă PDF |
||||||
| Rezumat | ||||||
Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results. |
||||||
| Cuvinte-cheie amniotic membrane, decellularization, morphology |
||||||
|
Crossref XML Export
<?xml version='1.0' encoding='utf-8'?> <doi_batch version='4.3.7' xmlns='http://www.crossref.org/schema/4.3.7' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xsi:schemaLocation='http://www.crossref.org/schema/4.3.7 http://www.crossref.org/schema/deposit/crossref4.3.7.xsd'> <head> <doi_batch_id>ibn-173107</doi_batch_id> <timestamp>1719095256</timestamp> <depositor> <depositor_name>Information Society Development Instiute, Republic of Moldova</depositor_name> <email_address>[email protected]</email_address> </depositor> <registrant>Asociaţia Stiinţifică Medicală din Modova</registrant> </head> <body> <journal> <journal_metadata> <full_title>Moldovan Medical Journal</full_title> <issn media_type='print'>25376373</issn> </journal_metadata> <journal_issue> <publication_date media_type='print'> <year>2022</year> </publication_date> <issue>2(65)</issue> </journal_issue> <journal_article publication_type='full_text'><titles> <title>Morphological evaluation of the amniotic membrane decellularization</title> </titles> <contributors> <person_name sequence='first' contributor_role='author'> <given_name>Olga</given_name> <surname>Ignatov</surname> </person_name> <person_name sequence='additional' contributor_role='author'> <given_name>Adrian</given_name> <surname>Cociug</surname> </person_name> <person_name sequence='additional' contributor_role='author'> <given_name>Oleg</given_name> <surname>Pascal</surname> </person_name> <person_name sequence='additional' contributor_role='author'> <given_name>Viorel</given_name> <surname>Nacu</surname> </person_name> </contributors> <publication_date media_type='print'> <year>2022</year> </publication_date> <pages> <first_page>30</first_page> <last_page>35</last_page> </pages> <doi_data> <doi>10.52418/moldovan-med-j.65-2.22.04</doi> <resource>http://www.crossref.org/</resource> </doi_data> </journal_article> </journal> </body> </doi_batch>
|
|
|
