Morphological evaluation of the amniotic membrane decellularization

Ignatov Olga; Cociug Adrian; Pascal Oleg; Nacu Viorel

Conţinutul numărului revistei

Articolul precedent

Articolul urmator

328

Ultima descărcare din IBN:
2023-08-31 21:46

Căutarea după subiecte
similare conform CZU

611.013.8 (6)

Anatomy. Human and comparative anatomy (239)

SM ISO690:2012

IGNATOV, Olga, COCIUG, Adrian, PASCAL, Oleg, NACU, Viorel. Morphological evaluation of the amniotic membrane decellularization. In: Moldovan Medical Journal, 2022, nr. 2(65), pp. 30-35. ISSN 2537-6373. DOI: https://doi.org/10.52418/moldovan-med-j.65-2.22.04

EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core

Moldovan Medical Journal

Numărul 2(65) / 2022 / ISSN 2537-6373 /ISSNe 2537-6381

Morphological evaluation of the amniotic membrane decellularization

DOI:https://doi.org/10.52418/moldovan-med-j.65-2.22.04

CZU: 611.013.8

Pag. 30-35

Ignatov Olga¹², Cociug Adrian², Pascal Oleg¹, Nacu Viorel²¹

¹ ”Nicolae Testemițanu” State University of Medicine and Pharmacy,
² Human Tissue Bank, Moldova

Disponibil în IBN: 30 ianuarie 2023

Descarcă PDF

Rezumat

Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.

Cuvinte-cheie
amniotic membrane, decellularization, morphology

Cerif XML Export

<?xml version='1.0' encoding='utf-8'?>
<CERIF xmlns='urn:xmlns:org:eurocris:cerif-1.5-1' xsi:schemaLocation='urn:xmlns:org:eurocris:cerif-1.5-1 http://www.eurocris.org/Uploads/Web%20pages/CERIF-1.5/CERIF_1.5_1.xsd' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' release='1.5' date='2012-10-07' sourceDatabase='Output Profile'>
<cfResPubl>
<cfResPublId>ibn-ResPubl-173107</cfResPublId>
<cfResPublDate>2022-08-01</cfResPublDate>
<cfVol>65</cfVol>
<cfIssue>2</cfIssue>
<cfStartPage>30</cfStartPage>
<cfISSN>2537-6373</cfISSN>
<cfURI>https://ibn.idsi.md/ro/vizualizare_articol/173107</cfURI>
<cfTitle cfLangCode='EN' cfTrans='o'>Morphological evaluation of the amniotic membrane decellularization</cfTitle>
<cfKeyw cfLangCode='EN' cfTrans='o'>amniotic membrane; decellularization; morphology</cfKeyw>
<cfAbstr cfLangCode='EN' cfTrans='o'><p>Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.</p></cfAbstr>
<cfResPubl_Class>
<cfClassId>eda2d9e9-34c5-11e1-b86c-0800200c9a66</cfClassId>
<cfClassSchemeId>759af938-34ae-11e1-b86c-0800200c9a66</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfResPubl_Class>
<cfResPubl_Class>
<cfClassId>e601872f-4b7e-4d88-929f-7df027b226c9</cfClassId>
<cfClassSchemeId>40e90e2f-446d-460a-98e5-5dce57550c48</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfResPubl_Class>
<cfPers_ResPubl>
<cfPersId>ibn-person-45928</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-32742</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-11464</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-11432</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfFedId>
<cfFedIdId>ibn-doi-173107</cfFedIdId>
<cfFedId>10.52418/moldovan-med-j.65-2.22.04</cfFedId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
<cfFedId_Class>
<cfClassId>31d222b4-11e0-434b-b5ae-088119c51189</cfClassId>
<cfClassSchemeId>bccb3266-689d-4740-a039-c96594b4d916</cfClassSchemeId>
</cfFedId_Class>
<cfFedId_Srv>
<cfSrvId>5123451</cfSrvId>
<cfClassId>eda2b2e2-34c5-11e1-b86c-0800200c9a66</cfClassId>
<cfClassSchemeId>5a270628-f593-4ff4-a44a-95660c76e182</cfClassSchemeId>
</cfFedId_Srv>
</cfFedId>
</cfResPubl>
<cfPers>
<cfPersId>ibn-Pers-45928</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-45928-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
<cfFamilyNames>Ignatov</cfFamilyNames>
<cfFirstNames>Olga</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-32742</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-32742-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
<cfFamilyNames>Cociug</cfFamilyNames>
<cfFirstNames>Adrian</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-11464</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-11464-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
<cfFamilyNames>Pascal</cfFamilyNames>
<cfFirstNames>Oleg</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-11432</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-11432-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2022-08-01T24:00:00</cfStartDate>
<cfFamilyNames>Nacu</cfFamilyNames>
<cfFirstNames>Viorel</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfSrv>
<cfSrvId>5123451</cfSrvId>
<cfName cfLangCode='en' cfTrans='o'>CrossRef DOI prefix service</cfName>
<cfDescr cfLangCode='en' cfTrans='o'>The service of issuing DOI prefixes to publishers</cfDescr>
<cfKeyw cfLangCode='en' cfTrans='o'>persistent identifier; Digital Object Identifier</cfKeyw>
</cfSrv>
</CERIF>