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SM ISO690:2012 UNCU, Andrei. Elaborarea și validarea metodei cromatografice de lichide de înaltă presiune pentru dozarea propiltiohinotiadiazolului. In: Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, 21-23 octombrie 2020, Chişinău. Chişinău: USMF, 2020, p. 685. |
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Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu” 2020 | ||||||
Congresul "Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”" Chişinău, Moldova, 21-23 octombrie 2020 | ||||||
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Pag. 685-685 | ||||||
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Background. Propylthiohinothiadiazole is a thiadiazole derivative with pronounced antimycobacterial action. Substance is researched in order to develop methods of analysis and standardization, analytical documents regulating quality for use as an active principle in the development of pharmaceutical forms. Objective of the study. Elaboration of the work technique and validation of the HPLC method for dosing propylthiohinothiadiazole. Material and Methods. 3 series of propylthiohinothiadiazole, internal reference standard of 2-(propylthio)-5H-[1,3,4]-thiadiazole[2,3-b]-quinazolin-5-one substance with concentration 99,98%, chromatographic system Shimadzu LC-20AD HPLC with UVVIS detector, acetonitrile and dimethylsulfoxide of purity grade “pro HPLC analysis” (Sigma Aldrih and Merck), bidistilled purified water. Results. Linearity is proved for concentrations of 5-30 μg/ml, the linear regression equation is y=36472x-44580, R²=0.9929. The limit of detection is 0,729 μg/ml and the limit of quantification is 2,210 μg/ml. It was established that the method is accurate (mean recovery values at 80%, 100% and 120% concentration levels were close to 100%. The accuracy of the method was expressed by repeatability and intermediate accuracy. The variation of the chromatographic conditions established that the method is robust. For all validation parameters, RSD was less than 1. Conclusion. The validation results show that the developed HPLC method is simple, fast, accurate and reproducible. |
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Cuvinte-cheie HPLC, propylthiohinothiadiazole, dosing, validation, HPLC, propiltiohinotiadiazol, dozare, validare |
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