Traditional microscopy is a powerful tool for imaging small nontransparent biological objects, such as seeds for example. However, anybody who has used one will know its limitations: a tiny field of view, a shallow depth of field and low balance between reflected/scattered light and incident one. Many of the biological objects are non specular. Moreover, often the raising the illumination intensity is strict limited as focused light of illuminator can destroy the biological sample by heating or by irreversible photostructural changing. There is a growing interest to get information on micro-scale of the biological objects, such as 3D surface morphology directed to make a careful study their biology.  Pointed above limitations make it hard to get a sense and take an image of the 3D shape of objects to studying the surface morphology.  Aim of present work: Design of optical digital microscope by which one can study the 3D surface of small (down to 300μm) biological nontransparent objects. Main ideas suggested for microscope design and optical scheme for this aim are: Digital holographic microscope DHM as a base of a design Darkfield in reflection by epi-illumination of laser light Infinity-corrected microscope Fiber-optic configuration Smart camera and digital image processing  Designed the DHM provided with the epi-objective for darkfield illumination by laser beam.    Our home-made DHM is based on some standard microscope parts, with a laser illumination. Scattered light by an object propagates to the microscope’s focal plane, where it interferes with the undiffracted portion of the beam. The resulting interference pattern is imaged by digital camera.  Results: Combining microscopy and DH offers the unique advantage of simultaneously capturing complete 3D information about the specimen, and under coherent illumination it has been investigated for continuous PC acquisition and identification of biological objects.