Yield stability depends on resistance of varieties and hybrids to stressful environmental factors. Assessment of genotype-environment interaction helps breeders select the best genotypes for submission to the state variety trials. The article presents results of evaluation of the genotype-environment interaction in 8 promising spring barley lines and 2 standard varieties by AMMI analyses. The analysis of variance on grain yield data showed mean squares of environments, genotypes and genotype-environment interaction (GEI) respectively accounted for 85,8, 8,1 and 6,1% of treatment combination sum of squares. To find out the effects of GEI on grain yield, the data were subjected to AMMI (Additive Main effects and Multiplicative Interaction) analysis. The AMMI model presented greater efficiency by retaining most of the variation in the first two main components – 95,7%. Lines 09-837 and 08-1385 presented an elevated grain yield and stability as determined by the AMMI method. These lines named as «Avhur» and «Veles» was submitted to the state variety trial. The results finally indicated that AMMI are informative method to explore stability and adaptation pattern of genotypes in practical plant breeding and in subsequent variety recommendations.