Ruta graveolens and Ampelopsis brevipedunculata were introduced in the Botanical garden, and studied their ontogenesis, and requirements for environmental conditions in the Lviv region. For both of introduced species have been identified opportunities of cultivation with “the principles of good practice cultivation and collection” and obtaining sufficient for using medicinal raw material. It has been studied the antibacterial activity of plant extracts on a number of bacteria of collection strains and isolated from the oral cavity of patients with periodontitis. It was discovered the activity of water extracts on Enterobacter cloacae (Enterobacter spp.) only, while the 70% ethanol-water extracts were active against Escherichia coli, Staphylococcus aureus, Streptococcus salivarius, Enterobacter spp., and Neisseria sp.