Development of digital holographic microscope for 3D sensing of biological surface morphology
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AKIMOVA, Elena. Development of digital holographic microscope for 3D sensing of biological surface morphology. In: IFMBE Proceedings: 3rd International Conference on Nanotechnologies and Biomedical Engineering, ICNBME 2015, Ed. 3, 23-26 septembrie 2015, Chişinău. Springer, 2016, Editia 3, Vol.55, pp. 398-403. ISBN 978-981287735-2. ISSN 16800737. DOI: https://doi.org/10.1007/978-981-287-736-9_95
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IFMBE Proceedings
Editia 3, Vol.55, 2016
Conferința "International Conference on Nanotechnologies and Biomedical Engineering"
3, Chişinău, Moldova, 23-26 septembrie 2015

Development of digital holographic microscope for 3D sensing of biological surface morphology

DOI:https://doi.org/10.1007/978-981-287-736-9_95

Pag. 398-403

Akimova Elena
 
Institute of Applied Physics, Academy of Sciences of Moldova
 
 
Disponibil în IBN: 17 iunie 2022


Rezumat

Sunflower broomrape Orobanche cumana Wallr.(O. cumana) is a parasitic angiosperm that infects the roots of sunflower plants, causing significant damages to this strategic oilseed crop. Combining microscopy and digital holography (DH) offers the unique advantage of simultaneously capturing complete three-dimensional (3D) information about the biological specimen, and under coherent illumination it has been investigated for computer acquisition and identification of biological specimens. Configuration of the digital holographic microscope optical setup provided with the microscope epi-objective for darkfield illumination by laser beam is described. The aim of this research is to develop microscopy 3D system to enhance the resolution and contrast in imaging of O. cumana seeds for they systemizing. Successful application of the modern digital holographic microscope allowed studying the broomrape seed in various layers, like surface and under surface of seed.

Cuvinte-cheie
Biomedical engineering, Computer generated holography, holography, Laser beams, nanotechnology