Drought resistance of plants is based on a complex of genetic, physiological, coenotic traits - and their interaction. Such complex trait is difficult to manipulate by breeders. Creation of drought resistant varieties requires the drought tolerant starting material. Therefore, careful researches on the drought resistance of plant genetic resources is very important. Screening of the collections of genetic resources requires rapid assessment of a large number of samples. The methodology for determining drought tolerance in the early vegetation phase by germinating in osmotic solutions is the most convenient, cheap and effective.   In articles on the assessment of drought tolerance by the method of germination on osmotic solutions, there is no uniformity and there is no method for selecting the concentration of working solutions. To determine the differentiating ability of various (5%, 10%, 15%, 20% 25%) PEG-6000 solution concentrations, we carried out the germination of four pea varieties from the collection of the National Center for Plant Genetic Resources of Ukraine (NCGRU). The following traits have been researched: Oplot and Camelot are grain semi-dwarf leafless; Kharkivsky Jantarny is grain medium-high leaflet variety, and Adahumsky is vegetable semi-dwarf leaflet variety. Samples were germinated in solutions of five PEG-6000 concentrations, control is distilled water at the 21° C. Assessment of seedlings was observed on the third day.  The result established that PEG-6000 25 % was lethal for whole accessions, with 20 % and 15 % concentrations only Camelot (35 % and 85 % accordingly, compared with control) was seedling. With 10 % osmotic concentration two pea varieties has seedling: Kharkivsky jantarny (68 %) and Camelot (100 %); with 5 % concentration Adahumsky and Camelot have seedling similar with control, Oplot – 75 % and Kharkivsky jantarny – 96 %. Due to the large differentiation in germination at different concentrations of these pea varieties, it became essential to determe a single solution concentration for further work with a large volume of the collection of pea genetic resources. It was necessary to calculate the LD50, i.e. such a concentration of osmotic, at which the average laboratory germination for 4 varieties is 50% (sprouted seeds), or close to it. Calculation of LD (50) was carried out in three ways: using unweighted probit analysis, weighted probit analysis, and the equation of the logistic curve (Verhulst curve). As a result, it was found that the average LD value (% of sprouted seeds) for 4 genotypes corresponds to 50% at a PEG-6000 concentration of 8.359%. At this concentration, the laboratory germination of the studied pea varieties will be: Oplot - 4.21%; Adahumsky - 5.21%; Kharkivsky jantarny - 92.79% and Camelot - 97.78%. Such indicators will be 99.6% of the maximum variance. Thus, a PEG-6000 concentration of 8.359% is the most differentiating one when assessing pea genetic resources for drought tolerance in the early stages of vegetation and can be recommended for further use in screening collection accessions.  This work continues on a larger sample of pea collection samples using a working solution concentration of 8.5% with possible further adjustments.