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SM ISO690:2012 AILIESEI, Gabriela, MACSIM, Ana-Maria, DORNEANU, Cristina, STAVARACHE, Cristina, TIRNACOP, Lacramioara, NICOLESCU, Alina Florica, DELEANU, Călin. Reproducibility assessment for NMR metabolomics. In: Achievements and perspectives of modern chemistry, 9-11 octombrie 2019, Chişinău. Chisinau, Republic of Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2019, p. 202. ISBN 978-9975-62-428-2. |
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Achievements and perspectives of modern chemistry 2019 | |
Conferința "International Conference "Achievements and perspectives of modern chemistry"" Chişinău, Moldova, 9-11 octombrie 2019 | |
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Pag. 202-202 | |
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NMR spectroscopy is the most informative spectroscopic technique for structure elucidation of liquid compounds or compounds in solution. However, the NMR technique is less sensitive than other spectroscopic techniques and, in the same time, it is more affected by experimental factors. These drawbacks delayed the introduction of fully automated NMR analyzers in comparison with analyzers based on techniques like LC/GC-MS. NMR metabolomics relies on high reproducibility of the recorded spectra and high stability of experimental conditions. Our group has a long lasting interest in NMR metabolomics applied to plant and body fluids. We have been involved in one of the first inter-laboratory trials on blood plasma, when this technique was in the early development stage (1996), and we have been recently conducted an intra-laboratory test on quantitation of metabolites in fruit juices (2013). Latest generation of NMR instruments provide higher stability and reproducibility of results opening new gates in plant and animal metabolism. We present here our preliminary results on an NMR inter-laboratory, multi-operator quantitation trial for metabolites in complex natural matrices. |
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