When extracting plant materials, soaking is recommended, however, air bubbles, clogging the capillars, do not fully give out the biologically active substances (BAS). Ultrasound (US), creating a sound-capillary effect, accelerates the displacement of these air bubbles and creates the conditions for dissolution of biologically active substances. Therefore, ultrasound allows not only intensifying the process of mass transfer but also reducing extraction time, and increase the yield of valuable components from tomato seeds. The possibility of using ultrasound to extract biologically active substances from tomato seeds from different regions of Moldova from waste from the production of tomato juice of vegetable raw materials was studied. Based on the studies, the following stages of two combination methods were proposed for extracting biologically active substances from tomato seeds: 1 - preliminary ultrasonic treatment of whole or crushed seeds; 2 - three-stage extraction of treated seeds, unlike four according to traditional technology. The main parameters of the process of ultrasonic processing are determined, allowing to predict the efficiency of the output of biologically active substances compared with traditional technology. It is shown that the combination of the traditional method of extraction with ultrasound for the extraction of valuable components from waste of plant origin is promising and competes with the traditional one by shortening the process time from 36 to 12 hours, lowering the temperature from 100 to 55oC and yield of biologically active substances up to 1.5 times.