Haploid technologies in maize breeding
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2023-03-19 22:23
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ROTARENCO, Valeriu, DICU, Georgeta. Haploid technologies in maize breeding. In: International congress on oil and protein crops, 20-24 mai 2018, Chişinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2018, p. 86. ISBN 978-9975-3178-5-6.
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International congress on oil and protein crops 2018
Congresul "International congress on oil and protein crops"
Chişinău, Moldova, 20-24 mai 2018

Haploid technologies in maize breeding


Pag. 86-86

Rotarenco Valeriu1, Dicu Georgeta2
 
1 Var Breeding Ltd,
2 Evo Plant Srl
 
 
Disponibil în IBN: 4 septembrie 2019


Rezumat

A genotype with an ability to induce maternal haploids in vivo, haploid inducer (Stock 6), was developed by Ed. Coe in 1959 and that was a breakthrough in the implementation of haploid technologies in maize breeding and research. However, using such technologies in large breeding programs in different environments has required a significant improvement of the inducers. The frequency of haploid induction in our new inducers is 15-20%, whereas in the initial inducers, Stock 6 and MHI (Chalyk, 1999), it is 1-2% and 6-8%, respectively. New inducers possess additional marker genes, Pl1 and B1, which simplify the identification of haploids at different growing stages. All important agronomic traits of the new inducers have also been improved. The doubled haploid (DH) technology has already become an important tool which accelerates the production of inbred lines in maize breeding – it takes only two seasons to obtain a completely homozygous line. We have managed to develop a rather efficient DH technique which is allowing us to produce hundreds of DH lines every year. In haploid plants, allelic gene interactions (dominance and overdominance) are lacking and that facilitates the identification of genotypes with favorable non-allelic gene effects. Based on this fact, we have proposed to use haploid plants in a recurrent selection scheme to improve breeding initial material - synthetic populations. The Haploid Recurrent Selection (HRS) is applied in two populations - SP and SA. After five cycles of HRS, the grain-yield increase per cycle was more than 10% in both populations - it is a significantly higher result in comparison with the other known recurrent selection schemes (2-4%) (Hallauer and Miranda, 2010). The SP population has reached the grainyield level of F1 hybrids used in maize production. On our opinion, the haploid technologies have a high potential both to accelerate the breeding process and increase its efficiency.