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SM ISO690:2012 CHEBAN, Anna, CURSHUNJI, Dmitrii. The content of total protein and fat in seeds, as well as some parameters of productivity in breeding materiel of chickpea (Cicer arietinum L.).. In: International congress on oil and protein crops, 20-24 mai 2018, Chişinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2018, p. 69. ISBN 978-9975-3178-5-6. |
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International congress on oil and protein crops 2018 | ||||||
Congresul "International congress on oil and protein crops" Chişinău, Moldova, 20-24 mai 2018 | ||||||
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Pag. 69-69 | ||||||
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The protein content is most important component for legumes in the biochemical aspect. Fat is important as the nutritional value and flavor [Tripathi Ajai et al., 2016]. The aim of present study was to determine the protein and fat content of 10 genotypes viz.: gulabi: C76/7a, C156/1, C156/2, C156/3, C156/5, C156/8, C187/1, C187/3 and C48/28 (desi), C90/6d (kabuli) in F7-generation and their general characterization. The experimental material is by origin from intraspecific hidrid combination ♀ MDI 02432 (desi, black seeds) × ♂MDI 02419 (kabuli, beige seeds). As check was used Botna (desi, brown) and Ichel (kabuli, beige) cvs. The amount of spare protein in the chickpea seeds was determined by the Bredford reagent with Coomassie Brilliant Blue G-250 dye, which does not bind to peptides and proteins of low molecular weight (below 3 kD), does not interact with free amino acids and reagents of non-protein components of analyzed material. For the extraction of vegetable fat, diethyl ether was used. The crude protein content varied within: 24.16% (C156/3, C156/5) - 28.34% (C90/cd). By standards Botna and Ichel protein content were amounted 26.85% and 23.47%, respectively. Genotypes C187/1(26.66%) and C187/3 (26.68%) for protein contain at the level of the Botna standard. Genotype C156/1 with value 25.84% and previous genotypes is exceeded the best parental form MDI 02432 (25.47%). Genotypes C156/3, C156/5 and C156/8 with value of protein content: 24.16%, 24.16% and 24.66% respectively, are at the level to the parent form MDI 02419 (24.28%). Genotype C90/6cd for protein content exceeds Ichel cv., MDI 02419, MDI 02432 and Botna cv. for 17,2%, 14,3%, 10,1% and 5,3% respectively. Genotype C76 /7a (26.28%) exceeds only 3.1% of MDI 02432, for 7, 6% MDI 02419 genotype and 10.7% standard Ichel. The fat content for the studied genotypes varied from 2.7 % (C90/cd) to 10.2 % (C156/3). By standards Botna and Ichel fat content amount: 6.56% and 6.26%, respectively. For parent forms: 4.9% (MDI 02419) and 7.8% (MDI 02432). The best values for these parameters were by following genotypes: C 156/2(9.0%), C156 /8(9.2%) and C156/3(10.2%). For level at the best parental form were genotypes: C156/5(7.3%) and C156 1(7.95%). The lowest fat content is 2.7 % in the C 90/cd genotype with the highest protein content. By genotypes C76/7a, C48/28, C187/1 and C187/3 fat content is varied within 3,6 % - 4,2 %. Weight of 100 seeds (mean) by genotypes – C187/1, C187/3 and C48/28 is amounted: 26.0g, 26.5g, and 28.5g, by genotypes from the group C156 amounted 26.130.0g, C90/cd (29.2g), C76/7a (28.1g). By Botna and Ichel cvs, value amounted 28.7g and 32.1g, for genotypes MDI 02432 (29.9g) and MDI 02419 (33.3g). On productivity (grain yield) the genotypes: C187/1, C156/2, C48/28, C 90/cd and C76/7a at the level or exceeded standards Botna and Ichel, the others exceeded for yield parent genetypes, but less productive than standards. |
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