The connection of hydrographic basins allowed the spread of many fish species from their native areas to adjacent territories where, most likely, they became the dominant invasive species. Prussian carp (Carassius gibelio, Bloch) is probably the dominant invasive species of the last decades in south-eastern europe and in some cases it has become the native species in many lakes across the Balkan Peninsula and Turkey. Regarding its origin, Carassius gibelio is considered to be native in the area between east-central europe and Siberia (libosvarsky 1961) or heavily introduced from eastern Asia (Asian taxon according for instance to Copp et al. 2005) in the 17th century (lever 1996).The invasive expansion of this species is considered to have taken place through the lower basin of the Danube in 1912, on one side continuing in Bulgaria and Greece since 1950 (economidis et al. 2000) through the network of its tributaries and all the way to the Thrace region of Turkey, three decades later (Baran and Ongan 1998) and on the other side, through the higher tributaries of the Danube, reaching the Czech Republic via the Morava and Dyje rivers. The aim of this study is to identify the migration routes of C. gibelio, by cytochrome oxidase’s subunit I and cytochrome b DNA sequencing.In this study, mitochondrial marker, cytochrome b was used to infer the life history of Prussian carp (C. gibelio) from Eurasia. Our analysis was able to detect a) population structure, genetic diversity and evolution; b) prediction of migration pattern in Asia and invasion in europe. Additionally, the C. gibelio biochemical markers (SOD, GPX and MDA), as indicators of the health and physiological status, were identified.Our results reveal for species C. gibelio a high diversity over the ranging area, but a low population diversity. The number of component haplotypes within a population is generally small, probably determinate by same haplotypes fixation like an adaptation process for hydro-climatic factors, although are some exception. The large number of European haplotypes specific to this geographical area and average pairwise differences can mean a relatively recent radiation, so a recent expansion in this area. The migration pattern of C. gibelio individuals across eurasia was infer by combine three matrix: one genetically and another two geographic. Our data suggest that C. gibeliowas introduced artificially in Europe, followed by invasion and in Asia he naturally spread. Regarding the biochemical markers, we evidenced a decrease of the SOD and GPX specific activities along with a decrease level of lipid peroxidation products (MDA level), suggesting a good environmental conditions for C. gibelio population within Prut River.Acknowledgements:The study was possible with the financial support of the Resources pilot center for cross-border preservation of the aquatic biodiversity of Prut River, code MIS-ETC 1150, Joint Operational Programme Romania-Ukraine-Republic of Moldova, 2007-2013, Joint Managing Authority: Romanian Ministry of Regional Development and Housing.