Introduction A Botanical Garden’s mission of conservation of plant diversity must refl ect both on spontaneous and exotic fl ora. Botanic gardens are cultivating diverse collections of plants, but historically, medicinal plants collections are the cradle of this kind of institution. Each individual plant from this category represents a strategic reserve that may proove usefull at any time. The effective utilisation of these collections for conservation, is dependent on the adoptation of improved professional standards in documentation and genetic management. Material and methods The material is represented by the plant collections from the Greenhouses of the Botanical Garden from Iaşi, collections that comprise approximately 2000 taxa, of wich 87 have applications in traditional medicine. The individuals included in this categories are carefully studied, in order to observe different aspects of their reproduction in controlled conditions and to accumulate experience in their agrotechnology. The botanical classifi cation is due to the current works in the fi eld and the medicinal use of the plants are taken from classical and recent works. Results and discussions Taxa with medicinal uses come from different areas of the world and belongs at 40 botanical families, most of them are angiosperms. Only two taxa are ferns and fi ve taxa belongs to gymnosperms group. Some of them are very important because are listed on IUCN red List and 15 of them are recommended by BGCI to take more attention. The protection of some of the plants presented in this paper is due to the internal or external laws produced in the recent years. The plants of the collection are constantly observed; peculiarities of their growth, development and reproduction in the period of their vegetation are studied. It has been found that the species wich grew poorly and appeared endangered in natural forest conditios may grow well under cultivated conditions. Conclusions As a result of our analysis we noticed that  in the Greenhouses of Botanical Garden from Iaşi  are hold 87 plant species of medicinal interest, grown in controlled conditions. Some of them are protected by international law and convention. Through adequate growing agrotehniques we have in view the multiplication of these taxa in order to provide a suffi cient fund that will allow carrying on research activities or utilisation in the international exchange of botanical material.