Introduction A.V. Fomin Botanical Garden of Taras Shevchenko National University of Kyiv is one of the oldest centers of introduction and conservation phytodiversity in Ukraine. The cultivation of the plants from different phytogeographical zones within the territory of A.V. Fomin Botanical Garden is available through the variety of physical and geographical conditions of the area. These conditions are optimal for the naturalization different plants species. The most of these species have took the strong phytocenotic positions within the native plant groups. Material and methods The comparative morphological and geographical method was used. The nvestigations and specifi cation of plants were realized with the approach by Protopopova (1), Cherepanov (2). Results and discussions The natural landscapes including industrial forms of farming transformation is the powerful factor in vegetation change especially within the city. Urbanization process leads to transformation of the natural and creation of new ecotopes. Such situation is common for Kyiv city within which territory A.V. Fomin Botanical Garden is situated. The introduced species impact on environment is unsolved problem including their composition and vegetation. The species composition of wild fl ora of A.V. Fomin Botanical Garden is 240 species of vascular herbaceous plants. The vegetationt beyond the exposure areas is a complex relationship of different ecotops: residues natural wild areas and antropogenic landscapes. Among natural areas are forests, meadows, steppes, etc. The total distribution of the different vegetations is: sporadically occurring 74 species, single – 44 , occasionally encountered – 83, frequently - 36. All these types are united under the families: Amaranthaceae, Apiaceae, Apocynaceae, Aristolochiaceae, Asteraceae, Boraginaceae, Euphorbiaceae, Fumariaceae, Lamiaceae, Papaveraceae, Plantaginaceae, Phytolaccaceae, Portulacaceae, Primulaceae, Ranunculaceae, Rhamnaceae, Rubiaceae, Scrophulariaaceae, Urticaceae, Violaceae. Conclusions The urban fl oras investigations are the integral part of a botanical garden study. The majority of fl ora fragments are especially concentrated in botanical gardens at the territories of megapolises. On the example of A.V. Fomin Botanical Garden is shown that botanical gardens have the signifi cant value and importance for the species and groups of plants conservation.