Secular trees are solitary trees or small groups of isolated trees, remarkable by their ages, sizes, beauty, rareness, or for that have witnessed historic events. A number of 433 new secular trees, from 158 different sites are protected by state (Law on State Protected Natural Areas Fund (nr.1538/ 25.02.1998). A national survey of secular trees has been realized during the period of 2010-2012 in the framework of UNDP-GEF Project “Improving Coverage and Management Effectiveness of the Protected Area System in Moldova” and as a result 433 secular trees from 158 different sites have been assessed. A new concept on how to describe secular trees from state protected areas has been developed, which include next descriptors: height (m), the circumference of the stem (the stem perimeter (cm), stem diameter (cm, measured at a height of 1.3 m), age (years), the diameter of the crown (m), crown height (m), the number of primary branches the number of dry branches, health, natural and anthropogenic impacts, recommendations for improvement of the condition of the tree. Secular trees were grouped in two major categories: native species, including (Quercus robur, Quercus petraea, Fagus sylvatica, Fraxinus excelsior, Acer campestre, Pyrus pyraster, Populus alba, Sorbus aucuparia,Sorbus torminalis,Tilia cordata,Ulmus carpinifolua) and non-native species, including (Abies nordmaniana, Aesculus hipocastanum, Celtis occidentalis, Corylus columa,  Gleditsia triacanthos, Quercus castaneifolia, Libocedrus decurrens,  Maclura pomifera Picea abies, Picea canadensis, Picea pungens, Pinus strobus, Pinus nigra, Platanus occidentalis, Sophora gaponica). Secular trees may be classified according to their values into four groups: long-lived trees, remarkable trees, ornamental trees and rare tree species. Most of secular trees belong to the first group of long-lived trees with estimated age older than 100 years. Next  trees are most representative and may have also an European value: pedunculate oak (Quercus robur) from Cobâlea vilage, pedunculate oak from Schinoasa (Chişinău), pedunculate oak near to Boldureşti, Leova, pedunculate oak close to Pârâta village (Dubăsari), pedunculate oak near to Gură Bâcului, pedunculate oak from Pârâta vilage, pedunculate oak close to Micăuţi village, pedunculate oak from reserve „Călărăşeuca”, pedunculate oak from Galoci forest, pedunculate oak nearby Zahorna village, Lipcani, pedunculate oak from Curchi Monastery, pedunculate oak close to Zahorna village, pedunculate oak nearby Morenii Noi village, pedunculate oak close to Giurgiuleşti, pedunculate oak from Petruşeni forest, pedunculate oak from reserve „Livada Turcească”, common ash (Fraxinus excelsior) from Vărzăreştii Noi village and American sycamore (Platanus occidentalis) nearby Păuleşti village. Secular trees may be grouped according to their health conditions into next five groups: healthy secular trees, secular trees with partially damaged crown, secular trees with substantially affected crown, secular trees with broken trunk (hollows, scars etc.) and fallen or dead secular trees. Based on made inventory, 135 secular trees were attributed to healthy trees and 167 secular trees belong to the group of trees with partially damaged crown. Consequently, 302 secular trees were validated and submitted to be included into the List of state protected secular trees. Additionally the present inventory revealed that 71 secular trees, previously included in the former List of state protected secular trees, are missing as being dead, fallen or damaged by fire. Moreover 56 secular trees have been substantially damaged and were proposed to be excluded from the List of state protected secular trees. The carried out inventory of secular trees revealed, that during the last 40 years, 71 secular trees disappeared and 56 have been considerably damaged.  33 new remarkable secular trees have been identified in 27 different sites the last years. Newly discovered secular trees have been proposed to be included into the List of state protected secular trees. Our laboratory continues to carry out exploration activities for remarkable secular trees in the Republic of Moldova.