The genus Vitis contains two subgenera: Euvitis Planch. (bunchgrapes) and Muscadinia Planch (muscadine grapes). Muscadinia grape is genetically and morphologically distinct from species within the genus Vitis. There is disagreement as to whether the differences between Muscadinia and Vitis warrant generic status or whether it is better to consider these taxa as subgenera. The most obvious genetic difference between these two taxa is the number of somatic chromosomes: Muscadinia Small. species has 2n=40, and Vitis L. species has 2n=38. Muscadinia species differs from Vitis species in its seeds, bark, tendril and cluster morphology and possesses high resistance to Uncinula necator (Schw.) Burr., Meloidogyne Goeldi, Xiphinema index Thorne and Allen, Xylella fastidiosa Wells and Daktulosphaira vitifoliae Fitch. et al. The introgression of resistance to disease and pest from Vitis rotundifolia Michx. into Vitis vinifera L. was confounded by the differences in chromosome numbers. The V. rotundifolia cultivars exhibited 88 unique alleles that were not present in a database of more than 600 V. vinifera cultivars (S.Riaz, A.C.Tenscher, B.P.Smith, D.A.Ng, and M.A.Walker, 2008). A long standing goal of both Euvitis and Muscadinia breeding programs has been development of hybrids between these two subgenera, combining fruit quality from V. vinifera with resistance and environmental adaptation of muscadines. However, efforts to breed muscadine grapes commenced in the early 1900s and have generated a large number of cultivars and a limited number of hybrids V. vinifera. The works on synthesis of grapevine genome were initiated by A.Wylie, 1868; 1871, Detjen, 1919; Olmo, 1986 (F1). R.Dunstan, in 1962-1964, has initiated works by creating hybrids DRX (2n=39) (F2). In our researches, on the basis of  hybrid DRX- 55, a new species of crop V. vinifera L. type, similar to that cultivated on proper roots, before Viteus vitifolii Shimmer (Fitch) (1868) appearing and being distiguished by hibridogenous origin and high degree resistance to phylloxera, has been synthesized. The authentic synthesis of genome by backcrossing DRX-55 x Aramon x V. riparia, including by backcrossing with parental species, the hybrids Seyve Villari, polyploid forms and varieties of V. vinifera L. has been initiated (F3). According to our investigations, we can emphasize that the process of genome synthesis was performed in ex situ conditions due to the interaction of internal and external factors with the following backrossing (DRX M3-232-S.V.12-309, DRX M3-90x S.V. 20-366) (F4). The next stage, on the basis of hybrids F4 with several backrossing DRX M4-510 x Moldova, DRX M4-520 x GM-325-58, DRX M4-520 x Crystal, 80 hybrids has been created (F5). In our study, the authentic hybrids of species V. vinifera L. (2n=38) and V. rotundifolia Michx. (2n=40) served as biological material (F5). In the generation F5, the synthetic species V. vinifolia Top., V. rotundifera Dad. and V. crucestiana Top. were considered the exponents of authentic new genome of grapevine. These species grow on proper roots, blossoming and fertilizing normally, because they possess a genome equal to 2n=38 and high degree resistance to Viteus vitifolii Shimmer (Fitch) - phylloxera (Topala et al.2012). The vinifera grapes broke bud and bloomed about a month earlier than the muscadine grapes, while the hybrids were in between the parents. These hybrids can be used as bridges to carry viticulturally important genes from V. vinifera to V. rotundifolia and vice versa, to transfer resistant genes from V. rotundifolia to V. vinifera grape. In fact, it was established that with the aid of distant hybridisation method, forms which superpose in a single genotype the quantity and quality of harvest of the species V.vinifera L. with high resistance of V.rotundifolia. Michx. can be created.