Catalase, an enzyme involved in the decomposition of the residual quantities of hydrogen peroxide, is used in a variety of technologies related to textile, chemical and food industries. This enzyme is most commonly used in textile industry removing hydrogen peroxide after the bleaching of cotton-containing fabrics. In the food industry catalase is used for cold sterilization of beer, beverage and dairy products and in agriculture, animal husbandry and medicine – as a factor increasing the organism resistance to extreme influences. In order to increase the productive capacity of the fungus Penicillium funiculosum CNMNFD- 11 deposited in the National Collection of Non-pathogenic Microorganism of the Republic of Moldova, as producer of catalase, for the first time were used nanoparticles of silver (NPAg), copper (NPCu), bismuth (NPBi) and zinc (NPZn). Water dispersed nanoparticles were added in fungus growth medium in various concentrations: NPAg – 10–9; 10–7; 10–5 mol /L; NPCu – 10–7; 10–5; 10–1 mg /L; NPBi – 10–7; 10–5; 10–1mg /L; NPZn 10–7; 10–5; 10–1 mg /L. According to the results of previous experiments with vegetable and cereal seeds, these concentrations of nanoparticles provided fungicidal and stimulating effect. Catalase activity in culture medium was assayed by titration. The amount of biomass was determined by gravimetric method. The average of three measurements was calculated for each of three replicates. Action of aqueous dispersions of NPAg. Silver nanoparticles at concentrations 10–9; 10–7; 10–5mol/L appeared to have a moderate stimulating effect on biomass accumulation and catalase activity of Penicillium funiculosum CNMN-FD-11. The catalase relative activity was respectively 100%, 104%, 112%; and biomass accumulation – 116%, 104%, and 112% compared to the control. Action of aqueous dispersions of NPCu. The catalase activity and the productivity of studied strain were not affected by the NPCu at the concentrations of 10–1 and 10–5 mg/L and inhibited by the same nanoparticles at the concentration of 10–7 mg/L (respectively, 80% and 72% compared to the control). Action of aqueous dispersions of NPBi. After the cultivation of Penicillium funiculosum CNMN-FD-11 with bismuth nanoparticles at the concentrations of 10–7; 10–5; 10–1mg/L, the catalase activity was respectively 157%, 129%, 152% and the quantity of biomass, respectively – 153%, 122%, 100% relative to control. The concentration of 10–7 mg/l NpBi had a stimulating effect on biomass accumulation and catalase activity of the studied strain. Action of aqueous dispersions of NPZn. Zinc nanoparticles at concentrations of 10–7; 10–5; 10–1mg/L, proved to be efficient: the catalase activity was respectively 124%, 110%, 105%; and the quantity of biomass, respectively – 140%, 131% and 150% relative to the control sample. Thus, water dispersion with low concentrations of silver, bismuth and zinc nanoparticles had a significant stimulating effect on the catalase activity and fungal biomass accumulation, while aqueous dispersion of copper nanoparticles – an inhibitory effect.