| SM ISO690:2012|
SAPUNOVA, L.; KULISH, S.; TAMKOVICH, I.; LOBANOK, A.. Comparative characterization of exohydrolases from bacillus amyloliquefaciens subsp. Amyloliquefaciens as potential feed additives. In: Microbial Biotechnology. Ediția 3, 12-13 octombrie 2016, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2016, pp. 152-153.
Ediția 3, 2016
Conferința "Microbial Biotechnology" |
Chișinău, Moldova, 12-13 octombrie 2016
Application of enzymes in stock breeding is determined by their ability to promote conversion and, consequently, nutritive value of fodder, leading ultimately to reduced cost and increased profitability of agricultural production. Of special demand now are multienzyme complexes. As a rule, they are composed by mixing constituent enzymes synthesized by diverse microorganisms or less frequently via generation of the whole spectrum of enzymes by a single strain-producer in the course of one process. Earlier we selected the strain Bacillus amyloliquefaciens subsp. amyloliquefaciens – a source of extracellular phytase. Depending on fermentation conditions this culture also synthesizes a complex of hydrolytic enzymes involved in degradation of proteins and polysaccharides, including hardly digestible celluloses, glucans, xylans. Aim of this study – production of partially purified exohydrolases of Bacillus amyloliquefaciens subsp. amyloliquefaciens, characterization of their properties and evaluation of hydrolytic action on recalcitrant plant materials. Ultrafiltration technique and subsequent protein fractionation with ethyl alcohol were used to derive enzyme preparations – в-glucanase, xylanase, cellulase, phytase, protease, б-amylase purified 5.25 to 21.60 times. It was found that the enzymes retain stability and display up to 80 % of catalytic activity in the range of temperatures 40–55 оС and рН 5.0–7.0 (see the figure). The impact of enzyme complex on various plant substrates (rye, oat, barley grain, soybean cake) results in increased ratio in their extracts of protein (1.3–3.6 times), reducing sugars (2.7–9.2 times), phosphorus (1.2–7.4 times) depending on type of the feedstock. The obtained experimental findings evidence attractive application prospects of enzyme complex from Bacillus amyloliquefaciens subsp. amyloliquefaciens as feed ration supplement and as catalyst promoting hydrolysis of vegetable feedstock to enhance nutritive and biological value of the resultant foodstuffs.