Mangroves are productive ecosystems, which contribute to the well being of different organisms. They are halotolerant small shrubs or trees on which the life of different species of birds, animals, crustaceans and molluscs are depended upon. Mangroves play an important role in maintaining the natural environment and different communities by providing resources like food, medicine and helping in mineral cycling, water purification, soil erosion prevention and minimizing the impact of natural disasters. The microorganisms found in mangroves are responsible for mineralization, biogeochemical cycling of nutrients and contribute significantly to the transfer of energy among various trophic levels. Most of these microbes and strains thereof produce large number of enzymes capable of hydrolyzing, oxidizing or reducing metabolic reactions. Cellulase is a type of hydrolyzing enzyme extracted from microorganisms that has applications in food, pharmaceutical, detergent and paper industries. The screening of bacteria was done from the mangrove in Payyanur region, Kerala. The physicochemical characteristics of the soil were analyzed, including soil type, temperature, pH, conductivity, organic carbon, phosphorous and potassium. Hydrolytic enzymes have a wide variety of applications in different industries such as food, pharmaceutical and textile. Four different colonies were obtained on zobell marine agar media through serial dilution plate count spread plate technique. The colonies were named as MSB-1, MSB-2, MSB-3 and MSB-4. We have examined their ability to produce hydrolytic enzymes, such as catalase, oxidase, amylase and protease, which were qualitatively analyzed in these four isolates. Hydrogen peroxide, Kovac’s oxidase reagent were used for catalase and oxidase assays, respectively. For amylase enzyme, isolates were cultured on starch agar medium and for protease on skim milk agar medium and incubated at 30°C for 48 hours. All four species of bacteria showed positive results towards catalase and amylase activity and also showed negative result for oxidase enzyme activity. All are capable of producing protease enzyme, except MSB-4. The colonies will be identified through molecular techniques for further study.