Investigation of non-traditional sources of bioactive substances is one of the current directions of biotechnology development. Cyanobacterium Spirulina platensis is widely explored and used in recent decades as a source of important bioactive substances. An important direction in biotechnology research is obtaining of spirulina biomass fortified with important micronutrients. Chromium(III) is an essential nutrient required for normal energy metabolism. In order to reduce chromium toxicity cyanobacterium Spirulina platensis can be cultivated in presence of coordination compounds of Cr(III) that obtaining biomass with high content of chromium and other bioactive substances with antidiabetic effect.  The purpose of the research was the study of chromium bioaccumulation in spirulina biomass.  Research subject served cyanobacterium Spirulina platensis CNM-CB-02. As regulators of chromium content in biomass were used coordination compounds of Cr(III) [K2Cr2(SO4)4]•12H2O and K2[Cr(NTA)(C2O4)(H2O)]•2H2O.  The maximal content of chromium from spirulina biomass, cultivated in presence of some Cr(III) coordinative compounds, was distributed in the fraction of glutelins and globulins (92,8mg% and 55,9mg% Cr, respectively for the compound [K2Cr2(SO4)4]•12H2O, and in the case of compound K2[Cr(NTA)(C2O4)(H2O)]•2H2O) - 128,6 mg% and 70,4mg% chromium, respectively).  The obtained chromo-component products can be recommended for insulin activity increasing in the cases of diabetes mellitus.