Problem statement: Formation of students’ psychophysiological readiness to their future professional work can be considered as one of the most important tasks of specialist training at universities. Approach: An important part of this is physical education and sports. Purpose: to study students’ results in physical education and sports, their attitude to a healthy lifestyle and physical readiness to their professional work. Results: physical activity of the overwhelming majority of students is insufficient. The percentage of smokers and alcohol users among students has been revealed. A number of gender differences have been identified: women-students do less physical exercises and sports than men-students; also women-students perform regular morning exercises less frequently and are less willing to participate in sportive events, but smoke and drink alcoholic beverages significantly less. The women-students have shown worse self-estimation of their working ability dynamics during a day and week, worse self-estimation of their health and a greater number of fatigue signs during university studies. The expressed positive influence of physical exercises and sportactivities on various aspects of students’ educational, psychological and social spheres, their working ability and health has been proved. Conclusions: The research is practically important for substantiation of physical education and sports application for formation of students’ psychophysiological readiness to their future professional activities.