Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development

Massai Diana; Bolesani Emiliano; Diaz Diana Robl; Kropp Christina; Kempf Henning; Halloin Caroline; Martin Ulrich; Braniste Tudor; Isu Giuseppe; Harms Vanessa; Morbiducci Umberto; Drager Gerald; Zweigerdt Robert

Conţinutul numărului revistei

Articolul precedent

Articolul urmator

681

Ultima descărcare din IBN:
2018-10-09 13:10

SM ISO690:2012

MASSAI, Diana, BOLESANI, Emiliano, DIAZ, Diana Robl, KROPP, Christina, KEMPF, Henning, HALLOIN, Caroline, MARTIN, Ulrich, BRANISTE, Tudor, ISU, Giuseppe, HARMS, Vanessa, MORBIDUCCI, Umberto, DRAGER, Gerald, ZWEIGERDT, Robert. Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development. In: Scientific Reports, 2017, vol. 7, p. 0. ISSN 2045-2322. DOI: https://doi.org/10.1038/s41598-017-04158-x

EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core

Scientific Reports

Volumul 7 / 2017 / ISSN 2045-2322

Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development

DOI:https://doi.org/10.1038/s41598-017-04158-x

Pag. 0-0

Massai Diana¹, Bolesani Emiliano¹, Diaz Diana Robl¹, Kropp Christina¹, Kempf Henning¹, Halloin Caroline¹, Martin Ulrich¹, Braniste Tudor¹², Isu Giuseppe¹³⁴, Harms Vanessa⁵, Morbiducci Umberto⁶, Drager Gerald¹⁵, Zweigerdt Robert¹

¹ Hannover Medical School,
² Technical University of Moldova,
³ University of Basel,
⁴ University Hospital of Basel,
⁵ Leibniz University Hannover,
⁶ Polytechnic of Turin

Disponibil în IBN: 2 februarie 2018

Vezi articolul

Rezumat

Controlled large-scale production of human pluripotent stem cells (hPSCs) is indispensable for their envisioned clinical translation. Aiming at advanced process development in suspension culture, the sensitivity of hPSC media to continuous peristaltic pump-based circulation, a well-established technology extensively used in hydraulically-driven bioreactors, was investigated. Unexpectedly, conditioning of low protein media (i.e. E8 and TeSR-E8) in a peristaltic pump circuit induced severe viability loss of hPSCs cultured as aggregates in suspension. Optical, biochemical, and cytological analyses of the media revealed that the applied circulation mode resulted in the reduction of the growth hormone insulin by precipitation of micro-sized particles. Notably, in contrast to insulin depletion, individual withdrawal of other medium protein components (i.e. bFGF, TGFβ1 or transferrin) provoked minor reduction of hPSC viability, if any. Supplementation of the surfactant glycerol or the use of the insulin analogue Aspart did not overcome the issue of insulin precipitation. In contrast, the presence of bovine or human serum albumin (BSA or HSA, respectively) stabilized insulin rescuing its content, possibly by acting as molecular chaperone-like protein, ultimately supporting hPSC maintenance. This study highlights the potential and the requirement of media optimization for automated hPSC processing and has broad implications on media development and bioreactor-based technologies.

Crossref XML Export

<?xml version='1.0' encoding='utf-8'?>
<doi_batch version='4.3.7' xmlns='http://www.crossref.org/schema/4.3.7' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xsi:schemaLocation='http://www.crossref.org/schema/4.3.7 http://www.crossref.org/schema/deposit/crossref4.3.7.xsd'>
<head>
<doi_batch_id>ibn-58946</doi_batch_id>
<timestamp>1714251760</timestamp>
<depositor>
<depositor_name>Information Society Development Instiute, Republic of Moldova</depositor_name>
<email_address>idsi@asm.md</email_address>
</depositor>
</head>
<body>
<journal>
<journal_metadata>
<full_title>Scientific Reports</full_title>
<issn media_type='print'>20452322</issn>
</journal_metadata>
<journal_issue>
<publication_date media_type='print'>
<year>2017</year>
</publication_date>
<issue></issue>
</journal_issue>
<journal_article publication_type='full_text'><titles>
<title>Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development</title>
</titles>
<contributors>
<person_name sequence='first' contributor_role='author'>
<given_name>Diana</given_name>
<surname>Massai</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Emiliano</given_name>
<surname>Bolesani</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Diana Robles</given_name>
<surname>Diaz</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Christina</given_name>
<surname>Kropp</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Henning</given_name>
<surname>Kempf</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Caroline</given_name>
<surname>Halloin</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Ulrich</given_name>
<surname>Martin</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Fiodor</given_name>
<surname>Branişte</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Giuseppe</given_name>
<surname>Isu</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Vanessa</given_name>
<surname>Harms</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Umberto</given_name>
<surname>Morbiducci</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Gerald</given_name>
<surname>Drager</surname>
</person_name>
<person_name sequence='additional' contributor_role='author'>
<given_name>Robert</given_name>
<surname>Zweigerdt</surname>
</person_name>
</contributors>
<publication_date media_type='print'>
<year>2017</year>
</publication_date>
<pages>
<first_page>0</first_page>
<last_page>0</last_page>
</pages>
<doi_data>
<doi>10.1038/s41598-017-04158-x</doi>
<resource>http://www.crossref.org/</resource>
</doi_data>
</journal_article>
</journal>
</body>
</doi_batch>