It is considered that the microorganisms, isolated from the outpatients receiving a treatment, are characterized by a lower resistance to antibiotics in comparison with the hospital stems, but there are the data that show the presence in the outpatient infections of the infectious agents that are resistant to the preparations of the first line and, sometimes, to the preparations of the second line. This article presents the results of the analysis of 3209 strains of microorganisms isolated from the patients with septic-purulent infections treated in hospital and the analysis of 731 strains from ambulatory patients. A higher variation of the species of microorganisms from the stationary patients (n = 44) compared to the ambulatory patients (n = 33) has been found as well as a higher resistance to antibiotics of strains of microorganisms isolated from the patients treated in hospital (53.64%) as opposed to the microorganisms isolated from the ambulatory patients (41.02%), (t = 10.68, p < 0.05). The difference of antimicrobial resistance between the strains in hospital and the strains isolated from the ambulatory patients, depending on the species of the microorganism and the group of antibiotics has been determined.