Vitamin C is probably one of the most highly well known vitamin, which plays an important role in many vital functions of human body. It contributes for the repair of skin, tendons, ligaments and blood vessels tissues. Vitamin C is also an important physiological antioxidant, which helps to prevent and treat scurvy, cold, cardiovascular diseases, cataract, cancer etc. For maintaining a good health, the Recommended Dietary Allowances for adults is 90 mg for male and 75 mg for female. Vitamin C is contained in many natural sources as fruits and vegetables, but it is highly susceptible to degradation, therefore it is contained in food supplements. European regulation provides that any supplement of vitamin C may be one of five compounds: L-Ascorbic Acid, Sodium-L-Ascorbate, Potassium-L-Ascorbate, Calcium-L-Ascorbate, and L-Ascorbyl-6-Palmitate. According to the legislation, in the Republic of Moldova, the state quality control of food supplements is not mandatory, being based on the quality control of producer. In this context, it becomes appropriate to determine the content of vitamin C in food supplements. The aim of the study was to evaluate the methods of quantitative determination of vitamin C. The PubMed, Medline, Cochrane, Embase and Springer databases were used to identify the specific methods of vitamin C using “vitamin C analysis” and “vitamin C quantitative determination” keywords. 73 bibliographic sources were eligible for our study. This review shows that redox iodometry and acid-base titrations were used in order to determine the content of vitamin C in food, drugs and some supplements. The iodometry was more accurate than alkalimetry in determination of vitamin C from samples that contain additional acids, which do not interfere with the oxidation of ascorbic acid by iodine. Less researches applied fluorimetric, spectrophotometric, high-performance liquid chromatography, enzymatic, kinetic and electrochemical methods. The iodometry and high-performance liquid chromatography are the most acurate methods of vitamin Ccontent determination. Pharmacy