The genus Helichrysum (Asteraceae) comprises about 600 species of perennial or annual plants distributed in Europe, Asia, and Africa, which are an abundant source of secondary metabolites, such as flavonoids, chalcones, phenolic acids, coumarins, pyrones, and terpenes [1]. In the Republic of Moldova only one species H. arenarium (L.) Moench-(popularly known as everlasting), occurs sporadically on the cliffs of the right bank of the Nistru River, in the landscape reserve "Climăuții de jos", in the Steppe Hills steppes, on the limestones of Naslavcea, with a declining population [2]. Another species H. italicum L. is recently introduced (2021) in the collection of the Scientific Practical Centre in the Field of Medicinal Plants of “Nicolae Testemițanu” State University of Medicine and Pharmacy. We proposed identification and assay of flavonoids from species of the genus Heichrysum (H. arenarium and H. italicum). Aerial parts and flowers of H. arenarium were collected from wild flora, and for H. italicum - from the collection. Flavonoids were identified by specific reactions as well as by thin layer chromatography. Determination of flavonoids was performed with aluminium chloride using a Metertech UV/VIS SP 8001 spectrophotometer. By colour reactions and CSS in the aerial parts and flowers of H. arenarium were identified: apigenin, luteolin, rutozide, quercetin, quercitrin, while in H. italicum: apigenin, rutozide, luteolin. The flavonoid concentration (mg/ml), expressed as rutin (λ=430nm) shows a higher content in the H. arenarii flores (1.46), followed by the H. italici flores (1.31), while the flavonoid content in the aerial parts is lower and varies from 0.80 for H. italicum to 0.89 for H. arenarium. Where the total flavonoid content (mg/ml), expressed as quercetin (λ=425nm) is higher and shows 10,69 in H. arenarii flores; 9,87 in H. italici flores, followed by aerial parts (2,76 for H. italicum to 3,62 for H. arenarium) with a maximum extraction in ethanol of 50% for all products.