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Ultima descărcare din IBN: 2023-09-13 17:23 |
SM ISO690:2012 BARB, Alina Cristina, PASCA FENESAN, Mihaela, PIRTEA, Marilena, MARGAN, Madalin-Marius, TOMESCU, Larisa Cristina, CEBAN, Emil, CIMPEAN, Anca-Maria, MELNIC, Eugen. Reassessing Breast Cancer-Associated Fibroblasts (CAFs) Interactions with Other Stromal Components and Clinico-Pathologic Parameters by Using Immunohistochemistry and Digital Image Analysis (DIA). In: Cancers, 2023, vol. 15, p. 0. ISSN 2072-6694. DOI: https://doi.org/10.1090/noti2749 |
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Cancers | |
Volumul 15 / 2023 / ISSN 2072-6694 | |
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DOI:https://doi.org/10.1090/noti2749 | |
Pag. 0-0 | |
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Background: Breast cancer (BC) stroma has CD34- and αSMA-positive cancer-associated fibroblasts (CAFs) differently distributed. During malignant transformation, CD34-positive fibroblasts decrease while αSMA-positive CAFs increase. The prevalence of αSMA-positive CAFs in BC stroma makes microscopic examination difficult without digital image analysis processing (DIA). DIA was used to compare CD34- and αSMA-positive CAFs among breast cancer molecular subgroups. DIA-derived data were linked to age, survival, tumor stroma vessels, tertiary lymphoid structures (TLS), invasion, and recurrence. Methods: Double immunostaining for CD34 and αSMA showed different CAF distribution patterns in normal and BC tissues. Single CD34 immunohistochemistry on supplemental slides quantified tumor stroma CD34_CAFs. Digital image analysis (DIA) data on CAF density, intensity, stromal score, and H-score were correlated with clinico-pathologic factors. Results: CD34/αSMA CAF proportion was significantly related to age in Luminal A (LA), Luminal B (LB), and HER2 subtypes. CD34_CAF influence on survival, invasion, and recurrence of LA, LB-HER2, and TNBC subtypes was found to be significant. The CD34/αSMA-expressing CAFs exhibited a heterogeneous impact on stromal vasculature and TLS. Conclusion: BC stromal CD34_CAFs/αSMA_CAFs have an impact on survival, invasion, and recurrence differently between BC molecular subtypes. The tumor stroma DIA assessment may have predictive potential to prognosis and long-term follow-up of patients with breast cancer. |
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Cuvinte-cheie breast cancer (BC), cancer-associated fibroblasts (CAFs), CD34_CAFs, digital image analysis (DIA), immunohistochemistry (IHC), tumor stroma, αSMA_CAFs |
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