The study aims at assessing the phenolic profile of three Artemisia species and their antioxidant and cytotoxic potential. Plant material consisted in A. annua, A. absinthium and A. lerchiana aerial parts harvested from the ex situ collection of Botanical Garden, Chisinau, R. Moldova. Methanolic extracts were investigated by HPLC-DAD [1]. Total phenolic content (TPC) was determined by Folin-Ciocalteu method. The antioxidant activity was assessed by DPPH and ABTS radical scavenging assays, reducing power and ferrous ion chelating assays. Cytotoxicity was determined in vivo on Triticum aestivum L. caryopses [2, 3]. Chlorogenic acid, caffeic acid, cynarin, hyperoside and isoquercitrin were the major phenolic compounds identified in Artemisia extracts. The highest TPC was determined for A. annua (24.85 mg/g dw), followed by A. lerchiana (10.43 mg/g dw) and A. absinthium (4.89 mg/g dw). The highest DPPH radical scavenging effect was determined for A. lerchiana extracts (EC50 values ranging from 27.96 ± 0.21 to 40.82 ± 0.89 μg/mL). A. annua exhibited the highest ABTS scavenging effects (EC50 values ranging from 12.76 ± 0.35 to 16.47 ± 0.32 μg/ mL) and ferric ions reducing activity (EC50 values range: 15.06 ± 0.01 – 21.96 ± 0.08 μg/mL). All Artemisia extracts exhibited low ferrous ions chelating effects. The treatment of wheat caryopses with Artemisia extracts did not inhibit the germination, growth and biomass accumulation. In conclusion, the phenolic content and the antioxidant effects of Artemisia extracts suggest their possible use in food and pharmaceutical industries. Acknowledgements: The work is financed through SCOPES program of SNF (Switzerland), Project no. IZ73Z0_152265 and supported by a grant of Ministry of R&I, Program 1 - Development of the National R & D System, no. 22PFE/2018.