The purpose of the research is the analysis of the pharmaceutical potential of two plant species, namely Amaranthus retroflexus and Scilla autumnalis, which are currently not exploited in this field. The main objectives of the study are: the extraction and quantification of certain secondary metabolites, the antioxidant activity, as well as the protein content belonging to the two chosen species. The biological material used consisted of 5 calluses of Amaranthus retroflexus and 5 calluses of Scilla autumnalis. Callus induction was carried out starting from fragments of somatic tissue obtained by in vitro regeneration of shoots from the seeds of the 2 species. The results of this study demonstrated that Amaranthus retroflexus and Scilla autumnalis could be used in the pharmaceutical industry due to their content in certa-in secondary metabolites of interest such as polyphenols, tannins, triterpenes but also primary metabolites such as proteins. Both species had a total lack or an extremely small amount of flavonoids demonstrated by the lack of specific color resulting from the analysis. The more abundant amount of polyphenols, triterpenes and proteins was identified in Scilla autumnalis and tannins were more abundant in the case of Amaran-thus retroflexus species. The antioxidant activity present in both studied species was highlighted by the TEAC method, low antioxidant activity by the CUPRAC method. DPPH and FRAP methods did not reveal antioxidant activity for these two types of callus.