Aim: To investigate methods of extraction of polyphenol compounds in the aerial parts of Galium verum using methods such as water bath, ultrasound assisted and magnetic stirring extraction. Methodology: Galii veri herba was harvested from the spontaneous flora of the Republic of Moldova in the Bugeac steppe in 2021, during the blooming period. Polyphenol compounds were extracted with 60% ethanol solution for 30 min at 80°C, respecting the ratio of 1:10 for all three methods: water bath with condenser, ultrasound assisted and magnetic stirring extraction. Total polyphenol content was determined spectrophotometrically, by six consecutive measurements according to the Folin-Ciocalteu method, at 765 nm, expressed as gallic acid equivalents (GAE, mg/100 g vegetal product), with statistical processing of the data. Results: It is known that the diversity of phenolic compounds found in vegetal products makes it difficult to develop a universal extraction method, and of the three methods mentioned above, ultrasound-assisted extraction has proven to be the optimal method for the extraction of polyphenols from G. veri herba (29.21 ± 0.04 GAE), followed by water bath with condenser (27.56 ± 0.03 GAE) and magnetic stirring extraction (28.18 ± 0.03 GAE) mg GAE /100g vegetal products. Conclusions: The results confirm that Galium verum can be used as a source of polyphenols, and the total polyphenol content depends on the extraction method, the nature and concentration of the solvent, and the nature of the vegetal product.