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SM ISO690:2012 TIMINA, Olga, TIMIN, Oleg, AUTOR, Nou. Hairy roots characteristics of vegetable pea’s morphotype with modified leaf and offspring of the 89/16 sugar line transformant. In: Plant Genetics, Genomics, Bioinformatics, and Biotechnology: (PlantGen2021), 14-18 iunie 2021, Novosibirsk. Novosibirsk, Russia: Институт Цитологии и генетики СО РАН, 2021, Ediția a 6-a, p. 214. ISBN 978-5-91291-056-2. 10.18699/PlantGen2021-000 |
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Plant Genetics, Genomics, Bioinformatics, and Biotechnology Ediția a 6-a, 2021 |
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Conferința "Plant Genetics, Genomics, Bioinformatics, and Biotechnology" Novosibirsk, Rusia, 14-18 iunie 2021 | ||||||
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Genetic transformation of the vegetable pea has been tested by three wild strains of |
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<?xml version='1.0' encoding='utf-8'?> <oai_dc:dc xmlns:dc='http://purl.org/dc/elements/1.1/' xmlns:oai_dc='http://www.openarchives.org/OAI/2.0/oai_dc/' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xsi:schemaLocation='http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd'> <dc:creator>Timina, O.O.</dc:creator> <dc:creator>Timin, O.I.</dc:creator> <dc:creator>Autor, N.</dc:creator> <dc:date>2021</dc:date> <dc:description xml:lang='en'><p>Genetic transformation of the vegetable pea has been tested by three wild strains of<br />Agrobacterium rhizogenes. The steadily growing hairy roots of the acacia-shaped mutant<br />were obtained free from the vector. PCR analysis showed the presence of rol C gene in<br />hairy roots with a total protein content of 46.38%. The amino acid score protein<br />identified seven essential amino acids. Tryptophan was absent in the culture, limiting the<br />fullness of the protein. The vegetable sugar pea transformant was received, the offspring<br />of which was also free from the vector. Plants PCR analysis showed the presence of rol<br />D gene in progeny, including the control variant. It is assumed that the control identified<br />the appropriate homolog as a possible result of natural transformation, which occurred<br />in natural conditions. The offspring of the transformant was characterized by both an<br />increase in overall antioxidant activity and heat resistance in temperature stress<br />(25°≤t≤49 °C), which was assessed by direct method for the absence of leaves necrosis<br />in comparison with control. Transformant’s offspring was characterized by smaller leafs<br />sizes and a decrease in the stem length, the lower bean height, the number of knots up to<br />the lower bean and the dry mass of the plant. The results indicate an increase in the rate<br />of reaction of the transformant offspring, which has become consistent with the<br />mesothermophyte (heat-loving) ecogroup in comparison with control. This adaptation<br />may be based on the active expression of the rol D gene, which is known to encode an<br />enzyme that synthesizes proline, an amino acid with antioxidant, anti-denature osmo-<br />regulating properties and membrane-protective properties that adapt to stress. It is<br />assumed that the mechanism of increasing the antioxidant activity of transformants is<br />associated with an increase in the number of copies of the rol D gene.</p></dc:description> <dc:identifier>10.18699/PlantGen2021-000</dc:identifier> <dc:source>Plant Genetics, Genomics, Bioinformatics, and Biotechnology (Ediția a 6-a) 214-214</dc:source> <dc:title>Hairy roots characteristics of vegetable pea’s morphotype with modified leaf and offspring of the 89/16 sugar line transformant</dc:title> <dc:type>info:eu-repo/semantics/article</dc:type> </oai_dc:dc>