The tubers of yam are rich in nutrients, which are not only used for medicine and food, but also used as industrial raw materials and feeds. They are important economic crops in my country. Calcium-dependent protein kinases (CDPKs or CPKs), as a type of calcium-sensing protein, not only play an important regulatory role in the process of seed formation and germination, but also determine the formation of organs, participate in tuber development, and regulate hormone responses etc., as well as increasing plant tolerance to light, heat, salt and other stresses, play an important role in plant growth and development and stress response. In this study, the cloned yam CDPK20 gene was used to construct the plant expression vector pPZP221-CDPK, which was transferred into tobacco using the Agrobacterium-mediated method to verify the function of the gene. The results showed that: CDPK20 was successfully integrated into the tobacco genome and expressed at the transcriptome level. Under heat stress (42℃, treatment for 8h), light (dark treatment for 24h), and salt stress (250mM NaCl watering), the stress resistance of transgenic tobacco with CDPK gene was significantly enhanced compared with wild-type tobacco. The above research results provide theoretical basis for further research on the mechanism of CDPK gene in plant growth and development, and at the same time provide theoretical reference and technical support for improving the quality of yam and increasing the yield of yam from the molecular level, and are of great significance for exploring the growth and development of yam and tuber enlargement . Chinese Library Classification Number: (to be filled in by the editorial department) Document identification code: A Article number: 0513-353X