The article investigates the practical aspects of the implementation of different Ukraine’s economic cooperation vectors. Regions of any country demonstrate certain social and economic potential that explainstheir active participation in inter-regional cooperation. The cooperation is implemented under conditions of growing globalization, global interdependence and of international division of labor. The extension of interregional cooperation can be possible with the help of numerous activities. Among them are the following: the extension of industrial cooperation hold by enterprises of different regions; development of joint investment projects; joint actions in the financial, consulting and marketing activities; systematic exchange of information about the needs of business entities in investment and creation of a national network of international transport corridors. For the further development of trade and economic relations, it is necessary to create favorable conditions for entering of Ukrainian exporters to the world markets, improving the legal basis of bilateral relations, expansion of opportunities in the existing and new markets, the intensification of possible areas of cooperation. In addition, it is important to create new instruments of cooperation with trading partners of the African continent.