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SM ISO690:2012 CHELU, Cristina, VARLAM, Carmen, TIȚESCU, Gheorghe, BUTNARU, Gallia. Diversitatea moleculară a două ecotipuri de Datura inoxia provenite din vestul şi estul României. In: Protecţia plantelor - realizări şi perspective, Ed. 57, 27-28 octombrie 2020, Кишинев. Кишинев: "Print-Caro" SRL, 2020, nr.57, pp. 20-24. ISBN 978-9975-3472-0-4. DOI: https://doi.org/10.53040/9789975347204.03 |
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Protecţia plantelor - realizări şi perspective nr.57, 2020 |
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Simpozionul "Protecţia plantelor - realizări şi perspective" 57, Кишинев, Moldova, 27-28 octombrie 2020 | |
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DOI:https://doi.org/10.53040/9789975347204.03 | |
CZU: 575.3:582.951.4 | |
Pag. 20-24 | |
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Molecular Diversity of two Ecotypes of Datura inoxia Originating from Western and Eastern Romania. To characterize genomic variation among genotypes, we have performed RAPD analysis using ten random primers. The results yielded 88 bands out of which 39 were polymorphic. The primers US1 and US7 showed 87.71% and 72.72% polymorphism respectively. The least polymorphism was shown by primer US9 (12.50%). The primer US15 did not produce any bands suggesting the absence of matching sequences in the genomic DNA. The dendrogram classified ecotypes into two clusters (A and B); cluster B possess three sub-clusters: B1 - Socodor 2; B2 - Flamura 1 and Flamura 2, and B3 - Flamura 3. Overall, the values of genetic similarity between ecotypes were low pointing out their particular origin and “evolution”. |
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Cuvinte-cheie Datura inoxia Mill., Romanian ecotypes, RAPD oligomers, genetic similarity |
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