Biologically active substances of natural origin are often use in medicine
and agriculture for combating different diseases caused by fungi, bacteria and
viruses. The grape seeds are a valuable source for obtaining enotannins, that are
natural compounds with phenolic structures and which possess remarkable
antioxidant properties.The enotannins obtained from grape seeds are natural
compounds with polyphenolic structure, possessing significant antioxidant
properties, but their use is difficult, since most of the enotannins are insoluble in
water. To improve the physicochemical, antioxidant and antibacterial properties
of the enotannins, it was amended their chemical structure. Enotannins were
selected from local sources, from seeds of white (Enotanin 2) and red grapes
(Enotanin 1 and 3). New hydrophilic products (Enoxil I, II and III) were
obtained by depolymerization of the macromolecular chains of the initial
enotannins through the oxidation process by hydrogen peroxide using.
To determine the antioxidant properties of the Enoxil compound it was
used the chemiluminescence method (CL), in the generating luminescence
system- Luminol-H2O2. It was prepared 0.1% solution of Enoxil, Luminol
(1 mM) and H2O2 (0.2 mM) and as a buffer it was used Tris-HCl pH 8.6. The
results of the research for antioxidant activity (AA %) assessment of Enoxil (I, II
and III) have demonstrated that the values are basically the same. They are quite
pronounced and are in the range from 93.16 to 95.58%.
The obtained compounds have to be studied to determine their antifungal,
antibacterial and pharmacological properties.
Acknowledgements
Authors are grateful for the financial support accorded by the European Union's Seventh
Framework Programme (FP7-PEOPLE-IRSES-2013) under the grant agreement no PIRSESGA-
2013-612484.
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