In recent time many chemists focus their research for obtaining the
biological active substances from natural raw material, which can be used
in an intact or chemically modified form in different areas of human
activities. The plants constitute true biochemical manufactories, which
synthesize both basic substances necessary for their survival and a wide
range of compounds as well, that can be extracted and used in medicine,
food industry, perfumery, textile industry etc. So, the research in the field of
bioactive extracted compounds from different vegetal raw material is
certainly actual and of a high significance. A valuable raw material for
obtaining this category of substances can be the sawdust from the wood
processing factories.
The experimental studies have the aim of selecting the extraction method
and establishing the optimal conditions of this process for obtaining a
maximal quantity of biological active substances. For the extracts obtained
from vegetable material, the statical (maceration and the periodical
leakage) as well dynamical methods (extraction in an ultrasonic field) have
been used. For establishing the optimal parameters of extraction process a
series of factors have been taken into consideration: solvent nature, weight
ratio: vegetal product-solvent, pH of the medium, agitation intensity and
time of extraction. As a result, a method of extraction of bioactive
substances in an ultrasonic field during 30 minutes at the temperature of 25
Celsius degree and the weight ratio of “vegetal product-solvent” 1:5 using
as a solvent the ethyl alcohol of 50%, has been selected.
The newly obtained compound has a low water solubility, which reduces
essentially its using potential. As a result of oxidation, the extract obtained
from forestry wastes is solubilized, this process being accompanied with

the formation of new organic compounds, containing carboxylic, peroxidic,
alcoholic and phenolic functional groups. The total contain of the acid
functional groups (carboxylic and phenolic) as well the antioxidant activity
of the modified compound have been determined. The analysis of the
obtained results allows to conclude that the new modified compound shows
interest for future microbiological and pharmacological research, due to its
high antioxidant activity, approximately AA=85% (DPPH test) and AA=55%
(ABTS test) for the 1% water solution, and a considerable amount of acid
functional groups (Ctotal/g=4,382).
Acknowledgements: Authors are thankful for the financial support
accorded by the European Union's Seventh Framework Programme (FP7-
PEOPLE-IRSES-2013) under the grant agreement Nr. PIRSES-GA-2013-
612484.