The organic pollution is the result of contamination of water with organic substances originating both from natural and anthropogenic sources. The polar nature of the water molecule and the hydrogen bonds are responsible for many of water's unique physical and chemical properties. The chemical and physical properties of water depend on the amount and composition of dissolved materials. Lower Danube Euroregion comprises problematic (floodable, land-instable, prone to pollution) zones and natural protected areas (Lower Prut Meadow, Lower Danube Meadow, Danube Delta) and a proper management of aquatic resources is imposed. The aim of this study was to investigate the chemical (e.g., chemical oxygen demand, ammoniacal nitrogen, nitrates, total phosphor and organic matter in suspension) status for water quality from different areas of Lower Danube Euroregion, Galati and Tulcea regions. The chemical quality of the investigated groundwater and surface water streams region indicate a low level of organic contamination. The nitrate nitrogen in the groundwater and surface water streams from the studied region does not reach alarming levels. However, since the risk of contamination is higher in groundwater than in surface water streams, the nitrate nitrogen registered in the groundwater samples was slightly higher than in the surface water samples. It was observed that the concentration of phosphorus was null in groundwater samples confirming that no recent contamination took place. Nevertheless, in rivers the concentration of phosphorus is slightly higher since aquatic organisms are more likely to be present. The chemical oxygen demand was similar in all samples reaching low levels. Also, the total suspended solids registered as expected almost null values in groundwater samples and positive values in surface water samples. In addition, the concentration of total suspended solids in the small rivers was found to be higher than in the big rivers as a result of the dilution rate. The results will serve as a background for future investigations and will be compared to the ones obtained for other monitoring periods.