Pe animale de laborator s-a modelat hepatopatia (HP) toxică prin administrarea de durată a CCl4. S-a stabilit că CCl4 a provocat tulburări pronunţate ale metabolismulu glucidic care s-au manifestat prin creşterea nivelului de metaboliţi – lactat, piruvat şi malat şi modificări ale activităţii enzimelor tisulare – creşterea activităţii lactatdehidrogenazei (LDH) şi reducerea funcţionalităţii principalelor dehidrogenaze ale ciclului Krebs – malatdehidrogenazei NADP-dependente (MDH-NADPd), izocitrat dehidrogenazei NADP-dependente (ICDH-NADPd) şi succinatdehidrogenazei (SDH). Administrarea compuşilor biologic activi (CBA) autohtoni – CMD-8, CMJ-23 şi CMJ-33, polizaharidelor sulfatate din spirulină şi a remediului BioR Fe-Se în HP toxică a exercitat influenţe importante atât asupra modificărilor conţinutului de metaboliţi, cât şi a dinamicului de activitate a enzimelor investigate. CBA luaţi în studiu au reuşit să reducă intensitatea efectelor toxice ale CCl4 asupra hepatocitelor prin tendinţa de normalizare şi chiar readucerea la valori normale a nivelului de metaboliţi glucidici, precum şi a funcţionalităţii enzimelor celulare studiate.

The experimental toxic hepatopathy (HP) was modeled by long term administration of CCl4. It was established that CCl4 caused pronounced disorders of carbohydrate metabolism manifested by the increased level of the metabolites – lactate, pyruvate and malate, and changes in tissue enzyme activity – the increased activity of lactate dehydrogenase (LDH) and the reduction of the functionality of the main dehydrogenases of the Krebs cycle – NADP-dependent malate dehydrogenase (NADP-MDH), NADP-dependent isocitrate dehydrogenase (ICDH-NADP) and succinate dehydrogenase (SDH). The administration of local biologically active compounds (BAC) – CMD-8, CMJ-23 and CMJ-33, of Spirulina Platensis sulphatated polysaccharides (SSP) and of the remedy BioR Fe-Se in the toxic HP exerted important influences both on the evolution of metabolites content changes and on the activity of the investigated enzymes. The tested BAC succeeded to reduce the intensity of the toxic effects of CCl4 on hepatocytes by inducing a tendency of normalization and even by restoring to normal levels of the carbohydrate metabolites content, as well as of the functionality of studied cellular enzymes.