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579.873.2:616.24-002.5(478) (1) |
Classification and systematics of microorganisms (184) |
Pathology of the respiratory system. Complaints of the respiratory organs (757) |
SM ISO690:2012 CHESOV, Elena, CIOBANU, Nelly. Molecular resistance characteristics of Mycobacterium tuberculosis complex lineages in the Republic of Moldova. In: Revista de Ştiinţe ale Sănătăţii din Moldova, 2022, nr. 3 An.1(29), p. 118. ISSN 2345-1467. |
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Revista de Ştiinţe ale Sănătăţii din Moldova | ||||||
Numărul 3 An.1(29) / 2022 / ISSN 2345-1467 | ||||||
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CZU: 579.873.2:616.24-002.5(478) | ||||||
Pag. 118-118 | ||||||
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Background. The Republic of Moldova (RM) is among the countries with the highest rate of multidrug-resistant tuberculosis (MDR-TB). Mycobacterium tuberculosis complex (MTBC) includes several genotypic lineages with potential differences in the molecular drug resistance (DMR) determinants. Objective of the study. To compare the spectrum of DMR between different MDR MTBC lineages circulating in the RM. Material and Methods. Whole genome sequencing (WGS) of a set of MTBC MDR strains stored in the Biobank of the National Tuberculosis Reference Laboratory of the RM during 2013-2018 was performed. Sequenced strains were randomly selected in equal proportion for each year of the study period. Based on the sequencing data, phylogenetic tree of the studied strains was generated, and the spectrum of DMR associated mutations was described. Results. The study included 288 MTBC MDR strains. According to WGS data they belonged to 2 genotypic lineages L2 - 43% and L4 - 57%. L4 was characterized by a higher clustering rate than L2 (63.4% vs 36.3%, p< 0.0001). The statistically significant main differences between L2 and L4 in terms of DMR determinants included mutations: katG S315T (79% vs 5.5%); katG S315T + fabG1-15c>t (10.5% vs 92.1%); embB M306V (37.6% vs 19.7%); embA upstream (11.8% vs 36.4%); rpsL K43R (76.6% vs 1.2%); rpsL K88R (8.9% vs 90.7%); fabG1 - 15c>t (20.3% vs 97.4%); thyA R222G (78.7% vs 12.5%); Rv2670c A5V (6.4% vs 81.3%) eis -12c>t (14.6% vs 96.6%). Conclusion. MTBC MDR strains in RM belong to 2 genotypic lines L2 and L4 with significant differences in clustering rate and DMR determinants. |
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Cuvinte-cheie MDR TB, genotypic lineages, Mutation, TB MDR, linii genotipice, mutaţii |
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