Tomato is the most important vegetable cultured under glasshouses growing usually in monoculture or as part of a narrow crop rotation. An important aspect of this intensive cropping system is control of pest and diseases. Nematode Meloidogyne sp. is the main cause of yield losses in the glasshouse crops as their populations can build up very quickly under the ideal conditions of temperature and humidity. Many soil microorganisms including the actinomycetes have the capacity to produce the various biological active substances with antibacterial and antifungal activities which negatively influence on growth and development of some dangerous plant parasites including nematodes. The purpose of this research is to study the biological activity of some new strains of Streptomyces sp. isolated from the soils of R.Moldova. The metabolites of 27 strains of streptomycetes were studied on their phytostimulating and nematicidal activities. The influence of Streptomyces sp. metabolites on the germination of tomato seeds (grades Leana and Novelty of Dniester) has been studied. It has been revealed, that seeds of grade Leana possess higher germination. Such strains as Streptomyces sp. 11, Streptomyces sp. 22, Streptomyces sp. 182 increased the length of rootlets and their weight on 15 - 20 % in comparison with the control. In laboratory conditions the influence of metabolites of these strains on the plant parasite nematodes (order Tylenchida, phylum Nematoda) has been checked. The negative influence on second stage of juveniles of root-knot nematodes has rendered the metabolites of strains Streptomyces sp. 11 and Streptomyces sp. 182, causing the wrinkle of nematode cuticle and the decrease of their activity.