Streptomycetes isolated from soil of Republic of Moldova were tested on their ability to synthesize soluble pigment on Czapek’s medium, oatmeal agar, starch-ammonia agar (SAA), potato glucose agar (PGA). Only Streptomyces sp. 11 synthesized soluble pigment with different colors on media oatmeal agar. However, Streptomyces sp. 22 and Streptomyces sp. 47 were able to synthesize soluble pigment only on SAA medium and oatmeal agar respectively. All studied strains have shown the abilities to accumulate biomass on complex medium M-I. Results show a range in amount from 5.38 g/l to 12.5 g/l air-dry biomass. Secondary metabolites of streptomycetes are antagonists of pathogenic bacteria, fungi and increase mortality of plant parasitic nematodes. Furthermore, all studied strains showed ability to suppress the growth of Botrytis cinerea (diameter of detained zones of growth – 11.0-16.0 mm) and Fusarium oxysporum. Determination of antifungal activity of the studied strains showed the slower growth caused by S. sp. 22 (zone 16.0 mm). Moreover, these strains obtain the antibacterial activities against Clavibacter michiganensis, Agrobacterium tumefaciens, Erwinia carotovora and Xanthomonas campestris. The most intensively antibacterial activities of these strains were against Xanthomonas campestris and Clavibacter michiganensis (delayed zone of growth 13.0-16.0 mm and 13.5-15.0 mm). Also the influence of exometabolites of Streptomyces sp.11 and Streptomyces sp.154 showed an increase in length of rootlets of tomato seeds, among all strains. The influences of secondary metabolites of all studied strains were effectively in different degrees on mortality rate of invasive stage juveniles of root-knot nematodes in greenhouse. The results showed that secondary metabolites of S. sp. 11, S. sp. 22, S. sp. 47, S. sp. 76 possessed the nematicidal effects on second stage juveniles of Meloidogyne incognita.