In the past years, the coordinative compounds of trace metals became of high interest due to their large-scale theoretical and practical implementation. Their favorable influence on biological systems as agents which increase productivity and stability of microorganisms – sources of bioactive substances, plant resistance to unfavorable natural conditions is well known. This paper presents the experimental data of the influence of three coordinative compounds of zinc, cobalt and copper on cellulase (β-glucosidase and endoglucanase) and xylanase biosynthesis in the fungal strain Aspergillus niger CNMN-10. The submerged cultivation of micromycete was carried out in Erlenmeyer flasks at 28- 30°C upon continuous shaking (180-200 rot/min.), up to 6-9 days. The nutrient medium included the following (g/L): beet pulp – 20.0; wheat bran – 10.0; KH2PO4 – 1.0; CaCl2·2H2O – 0.1; KCl – 0.1; MgSO4·7H2O – 0.3; NaNO3 – 2.5; FeCl3 – 0.01; pH – 5.5-6.0. The coordinative compounds ([Zn2(CH3COO)4(NioxH2)2(bpy)(H2O)2] (1); [Co2(DH)4Cl2(bpe)] (2) and [Cu2(DH)4(bpe)] (3)) were added to the nutrient medium in the concentrations of 1, 5 and 10 mg/L. The medium without addition of coordinative compounds served as reference. It was established that coordinative compounds do not affect the biosynthesis of βglucosidase. The enzymatic activity was similar to that of control, varying in the range of 2,47-2,62 U/mL – in experimental variants and 2,51 – 2,59 U/mL – in control, respectively. The highest endoglucanase activity was found in the variant cultivated in presence of coordinative compound 3, at concentration 5 mg/l. The endoglucanase activity determined was 8.81 U/mL whereas it was 8.00 U/mL in the reference media. Additionally, the presence of this compound maintains the micromycete enzymatically active for a longer time. Thus, the endoglucanase activity determined on the 9th day of cultivation was 8,16 U/mL compared to 76,5 U/mL in reference/control medium. Long-term activity of the culture during its stationary phase is a beneficial trait for biotechnology. Addition of the compounds 1 and 2 did not influence the activity of the endoglucanase. These COP could be of the higher interest as stimulators of other group of hydrolytic enzymes – xylanase. The peak of xylanase activity in the presence of COP was observed already on the 6th day of cultivation and was 97.48 U/mL (1), 107.93 U/mL (2) and 104.45 U/mL (3) compared to 97.44 U/mL in the control on the 7th day of cultivation, which corresponds to its highest biosynthetic activity. Thus, the addition of COP into the cultivation medium accelerates the rate of xylanase biosynthesis by 24 hours. The duration of active synthesis phase in the presence of COP 2 at concentrations 1 - 5 mg/L was 72 hours, and it corresponded to 6 - 8th days of its cultivation. At addition of COP 3 the active synthesis phase lasted 48 - 72 hours depending on concentration, whereas the enzymatic activity was 113,52- 118,81 U/mL compared to 97,44 U/mL in the control. Thereby, the COP tested can be recommended as stimulators of xylanases biosynthesis in Aspergillus niger CNMN-10 known as a producer of cellulases and xylanases. COP 3 can be recommended as enhancer of the endoglucanase activity, by 10,12 % at the 8th day of cultivation, and xylanase activity by 21,93 % at the 7th day of cultivation.