Articolul precedent |
Articolul urmator |
516 1 |
Ultima descărcare din IBN: 2023-03-08 13:51 |
SM ISO690:2012 FEKLISTOVA, Iryna, HAM, Le Huy. 1-oxyphenazin induces systemic resistnace of tomato (SOlanum Lycopesicum) and cucumber (Cucumis Sativus) to alternaria. In: Biotehnologii avansate – realizări şi perspective: Simpozionul ştiinţific naţional cu participare internaţională, 24-25 octombrie 2013, Chişinău. Chișinău, Republica Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2013, Ediția III-a, p. 102. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Biotehnologii avansate – realizări şi perspective Ediția III-a, 2013 |
||||||
Simpozionul "Biotehnologii avansate – realizări şi perspective" Chişinău, Moldova, 24-25 octombrie 2013 | ||||||
|
||||||
Pag. 102-102 | ||||||
|
||||||
Descarcă PDF | ||||||
Teza |
||||||
Upon indoor growing of vegetables the ground and construction contamination appears to be very high by the end of annual rotation. So before starting new rotation, the soil replacement or disinfection is required. According to the data of some researchers, while analysis of the soil after disinfection revealed that as a rule the foci of Fusarium oxysporum remain in underground layers and microbial complexes primarily consisting of saprotroph species Doratomyces stemonitis, Humicola grisea, Papulospora pannosa, Pythium spp. and other pathogens are also discovered. As the soil gets cold, the fungi grow out from lower horizons to upper ones (disinfected). During 1.5–2 months, the steamed soil remains phytotoxic that is manifested by dyewood of plants. The use of plant systemic phytopathogen resistance inducers (so called elicitors) is a new milestone in development of the ecologically safe complex protection of the plants from various diseases. The plants treated with inducing agents at the early development stages activate multiple defence responses expressed in formation of chemical and physical barriers on the way of pathogen penetration and development. Thus, the plants obtain induced systemic resistance to biotic and abiotic factors. This paper deals with induction of tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) resistance to blackspot causal agent using phenazine antibiotics - 1 - oxyphenazin synthesized by rhizosphere bacteria Pseudomonas aureofaciens KMBU phz 127/11. We used the strain P. aureofaciens KMBU phz 127/11 resulted from chemical mutagenesis of strain P. aureofaciens KMBU phz 127 extracted from bed silt of Halong Bay (South China Sea, Vietnam) with further sampling for resistance to toxic analogue of metabolites of “aromatic pathway” – 6-diazo-5-oxo-L-nor-leucine. Mass-spectrophotometric analysis of the substance extracted from culture liquid of bacteria P. aureofaciens KMBU phz 127/11 revealed the presence of 1-oxyphenazin – C12H7N2OH (molecular weight 196 Da, maximum absorption corresponds to 260 and 387 nm). The level of 1-oxyphenazin synthesis using the strain under study was 180 mg/l. Elicitor activity of 1-oxyphenazin synthesized by P. aureofaciens KMBU phz 127/11 was examined in plants of tomato and cucumber extensively cultivated in the Republic of Belarus upon indoor cultivating. In order to study the ability of bacterial metabolites to induce the systemic resistance of plants, we have chosen the system of germ artificial inoculation with spores of plant pathogenic fungi Alternaria cucumerina in cucumber and Alternaria solani in tomato. To control the nature of infection, the microscopy observation of plant leaves inoculated with A. cucumerina and A. solani was made. It has been established that cucumber and tomato plants are able to form the systemic resistance in response to soil treatment with the tested elicitor. Introduction of 1-oxyphenazin 30 μg/ml into the plant rhizosphere subsequently inoculated with spores of Alternaria, blackspot causal agent, resulted in reduction of the number of the infected plants. Efficiency of phenazine preparation application on disease protection for tomato and cucumber was 51.07% and 49.13%, respectively. It is known that cascade of defence reactions triggered upon systemic resistance induction includes generation of active oxygen forms, accumulation of phytoalexin and accumulation or activity improvement of specific hydrolytic enzymes (for example, chitinase and glucanase). Since phenazine antibiotics are able to influence on free radical processes upon interaction with a cell, we have made an assumption that the resistance to blackspot causal agent in tomato and cucumber described in the paper is due to the systemic resistance induction . |