The genus Brassica has a long history of world wide cultivation and comprises a large and diverse group of important vegetable, oil, fodder and condiment crops. One of the species of the genus is Brassica rapa L., whichis a very diverse species with the longest cultivation history, and encompasses leafy vegetables, turnips, and oils. B.rapa morphotypes, including leafy vegetables, turnips and oil types, differ based on which organs are consumed as food. So, the question on quality of B. rapa plants one of the main questions of modern genetics and breeding, including technology of plant genetic resources valorification. Quantitative Trait Loci (QTL) analysis has been used to identify genes related to a wide range of phenotypic and biochemical traits of quality in Brassica rapa L. Two DH mapping populations resulted from crosses between Yellow sarsonYS-143, Pak choi РC175, and Vegetable turnip VT115 accessions (DH38: РC175 x YS143 and DH30: VT115 x YS143) have been used at present work. Both populations were evaluated for morphological and biochemicalcharacters ofqualityas lamina length, width, color, hairiness, surface, petiole length and width, as well as content of ascorbic acid, β-carotene, chlorophyll a + b, and total protein, respectively. Transgression beyond the parental values within the analyzed populations was observed for most of the measured morphological traits including those for which parental values hardly differed, such as leaf width and turnip traits.A total of 45 QTL affecting 7 morphological traits were detected. Principal component analysis and co-localization of QTL indicatedthat some components of the genetic control of leaf and petiole-related traits might be the same. The proportion of total variation explained by each QTL ranged from 5.1% to 37.1%. Two different parents contributed alleles with effects in both directions to most of investigated traits.Seven genomic regions affected two or more leaf traits, where lamina width co-segregated with other leaf traits. QTLs on R03, R05, R07, R09 и R10 were detected in both mapping populations, related to multiple traits, and appeared to be the major QTL affecting leaf size in the used populations. A total of 23 QTL affecting mentioned above biochemical component contents in B. rapa plans were detected in the used populations, most of them located on linkage groups R03, R05, R07, and R09. Some QTL affecting a same trait were detected in different populations in the same linkage group. A large percentage of phenotypic variation (38.7%) was explained by a QTL of ascorbic acidcontenton the higher middle of R07, which was detected in DH30. For the other three traits (β-carotene and chlorophyll a + b) in DH-38 and DH-30 populations, the additive effects of QTL accounted for 26.7%, 36.0% and 48.7%, respectively. The phenotype variation for total protein trait it was 15.5%. The locations of all significant QTL and their support intervals are discussed. Obtained linkage maps were compared, based on the common SSR or AFLP markers. Some QTL affecting a same trait were detected in different populations in the same linkage group (R03, R05 and R07). However, we could not confirm whether theseQTL have identical position because of lack of common markers in the genome regions of both mapping populations, where these QTL were detected.Some QTL, where the different parental alleles had either both positive or opposite effects on the traits were detected for β-carotene and chlorophyll a + b content.It must be emphasized that colocation of QTL may indicate that a single gene underlies the QTL, or implies that different but closely linked genes are involved. Reviled molecular markers can serve as effective tool upon mass screening of collection and breeding material for morphological and biochemical traits of quality. The work is supported by grant of RFBR 13-04-00128-a.